Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Phenanthrene contamination and ploidy level affect the rhizosphere bacterial communities of Spartina spp

Abstract : Spartina spp. are widely distributed salt marsh plants that have a recent history of hybridization and polyploidization. These events have resulted in a heightened tolerance to hydrocarbon contaminants, but the effects of this phenomenon on the rhizosphere microbial communities is unknown. Here, we grew two parental Spartina species, their hybrid and the resulting allopolyploid in salt marsh sediments that were contaminated or not with phenanthrene. The DNA from the rhizosphere soil was extracted and the bacterial 16S rRNA gene was amplified and sequenced, whereas the abundances of the genes encoding for the PAH ring-hydroxylating dioxygenase (RHD) of gram-negative and gram-positive bacteria were quantified by real-time PCR. Both the contamination and the plant genotype significantly affected the bacterial communities. In particular, the allopolyploid S. anglica, harbored a more diverse bacterial community in its rhizosphere. The interspecific hybrid and the allopolyploid also harbored significantly more copies of the PAH-RHD gene of gram-negative bacteria in their rhizosphere than the parental species, irrespective of the contamination treatments. Overall, our results are showing that the recent polyploidization events in the Spartina affected its rhizosphere bacterial communities, both under normal and contaminated conditions, possibly increasing its phytoremediation potential.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-02929499
Contributor : christelle allard Connect in order to contact the contributor
Submitted on : Wednesday, March 3, 2021 - 2:18:27 PM
Last modification on : Friday, June 3, 2022 - 3:27:39 AM

File

Cavé-Radet-2020-Phenanthrene ...
Files produced by the author(s)

Identifiers

Citation

Armand Cavé-Radet, Sara Correa-Garcia, Cécile Monard, Abdelhak El Amrani, Armel Salmon, et al.. Phenanthrene contamination and ploidy level affect the rhizosphere bacterial communities of Spartina spp. FEMS Microbiology Ecology, Wiley-Blackwell, 2020, 96 (10), ⟨10.1093/femsec/fiaa156⟩. ⟨hal-02929499v2⟩

Share

Metrics

Record views

88

Files downloads

108