Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Aspergillus Is Inhibited by Pseudomonas aeruginosa Volatiles

Abstract : Background: Pseudomonas aeruginosa (Pa) and Aspergillus fumigatus (Af) compete with each other for nutrients and survival in natural environments, and have been extensively studied because of their intermicrobial interactions in the human microbiome. These are the principal microbes infecting immunocompromised patients and persons with cystic fibrosis, particularly the airways. These intermicrobial studies have largely been conducted in liquid medium or on agar, and thus focus on soluble or diffusible microbial products. Several key inhibitory molecules were defined in such studies. Methods: in the present report, we examine several methodologies which can be conveniently used to study the interaction of microbial volatiles, including capture methods and kinetics. Results: Pa volatiles inhibit Af, and the inhibitory mechanism appears to be the incorporation of the inhibitory molecules into the substrate nourishing the Af, rather than directly onto Af structures. We define by mass spectroscopy some specific volatile Pa products that can inhibit Af. Some of these molecules are selected for interest by the study of gene deletion mutants, producing a few Pa strains that were impaired in inhibition. We presumed the volatiles of these latter strains could be excluded from the search for inhibitors. Conclusion: the Pa inhibition of Af via a gaseous phase could be critical components in their competition, particularly in airways, where more direct contact may not be extensive.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-03590599
Contributor : Michel Courcelles Connect in order to contact the contributor
Submitted on : Monday, February 28, 2022 - 4:29:55 PM
Last modification on : Tuesday, March 1, 2022 - 3:07:59 AM
Long-term archiving on: : Sunday, May 29, 2022 - 7:40:18 PM

File

jof-06-00118-v2.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Hasan Nazik, Gabriele Sass, Eric Déziel, David A. Stevens. Aspergillus Is Inhibited by Pseudomonas aeruginosa Volatiles. Journal of Fungi, MDPI, 2020, 6 (3), pp.118. ⟨10.3390/jof6030118⟩. ⟨hal-03590599⟩

Share

Metrics

Record views

31

Files downloads

5