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Article Dans Une Revue Cellular Physiology and Biochemistry Année : 2015

Embelin-Induced Phosphatidylserine Translocation in the Erythrocyte Cell Membrane

Ghada Bouguerra
  • Fonction : Auteur
Omar Aljanadi
  • Fonction : Auteur
Rosi Bissinger
  • Fonction : Auteur
Salem Abbes
  • Fonction : Auteur
  • PersonId : 941179
Florian Lang
  • Fonction : Auteur
  • PersonId : 882057

Résumé

Background/Aims: The antihelminthic, contraceptive, anti-inflammatory and anticancer phytochemical embelin is at least in part effective against malignancy by inducing suicidal death or apoptosis of tumor cells. Erythrocytes are similarly able to enter suicidal death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface. Signaling of eryptosis includes increase of cytosolic Ca2+-activity ([Ca2+](i)), ceramide formation, oxidative stress as well as activation of p38 kinase and protein kinase C (PKC). The present study tested, whether and how embelin induces eryptosis. Methods: Phosphatidylserine exposure at the cell surface was estimated from annexin V binding, cell volume from forward scatter, [Ca2+](i) from Fluo3-fluorescence, ceramide abundance utilizing specific antibodies and reactive oxygen species (ROS) from 2', 7'-dichlorodihydrofluorescein diacetate (DCFDA) fluorescence. Results: A 48 hours exposure of human erythrocytes to embelin (>= 25 mu M) significantly increased the percentage of annexin-V-binding cells and hemolysis. Embelin did not significantly modify [Ca2+](i). The effect of embelin on annexin-V-binding was not blunted by removal of extracellular Ca2+, by p38 kinase inhibitor SB203580 (2 mu M) or by PKC inhibitor staurosporine (1 mu M). Embelin did, however, significantly increase the ceramide abundance. Conclusions: Embelin stimulates phospholipid scrambling of the erythrocyte cell membrane, an effect involving ceramide formation. (C) 2015 The Author(s) Published by S. Karger AG, Basel

Dates et versions

hal-01358501 , version 1 (31-08-2016)

Identifiants

Citer

Ghada Bouguerra, Omar Aljanadi, Rosi Bissinger, Salem Abbes, Florian Lang. Embelin-Induced Phosphatidylserine Translocation in the Erythrocyte Cell Membrane. Cellular Physiology and Biochemistry, 2015, 37 (4), pp.1629-1640. ⟨10.1159/000438529⟩. ⟨hal-01358501⟩

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