Lebecetin, a C-type lectin, inhibits choroidal and retinal neovascularization

Abstract : Angiogenesis is a cause of visual impairment and blindness in the wet form of age-related macular degeneration and in ischemic retinopathies. Current therapies include use of anti-VEGF agents to reduce choroidal neovascularization (CNV) and edema. These treatments are effective in most cases, but spontaneous or acquired resistance to anti-VEGF and possible adverse effects of long-term VEGF inhibition in the retina and choroid highlight a need for additional alternative therapies. Integrins alpha v beta 3 and alpha v beta 5, which regulate endothelial cell proliferation and stabilization, have been implicated in ocular angiogenesis. Lebecetin (LCT) is a 30-kDa heterodimeric C-type lectin that is isolated from Macrovipera lebetina venom and interacts with alpha 5 beta 1-and alpha v-containing integrins. We previously showed that LCT inhibits human brain microvascular endothelial cell adhesion, migration, proliferation, and tubulogenesis. To evaluate the inhibitory effect of LCT on ocular angiogenesis, we cultured aortic and choroidal explants in the presence of LCT and analyzed the effect of LCT on CNV in the mouse CNV model and on retinal neovascularization in the oxygen-induced retinopathy model. Our data demonstrate that a single injection of LCT efficiently reduced CNV and retinal neovascularization in these models.
Type de document :
Article dans une revue
FASEB Journal, Federation of American Society of Experimental Biology, 2017, 31 (3), pp.1107-1119. 〈10.1096/fj.201600351R〉
Liste complète des métadonnées

Littérature citée [48 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01513615
Contributeur : Institut Pasteur Tunis <>
Soumis le : mercredi 3 mai 2017 - 13:29:32
Dernière modification le : mercredi 21 mars 2018 - 18:57:36
Document(s) archivé(s) le : vendredi 4 août 2017 - 12:52:38

Fichier

Montassar_2017_Lebecetin,_a_C-...
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Fadoua Montassar, Marie Darche, Amandine Blaizot, Sébastien Augustin, Jean-Baptiste Conart, et al.. Lebecetin, a C-type lectin, inhibits choroidal and retinal neovascularization. FASEB Journal, Federation of American Society of Experimental Biology, 2017, 31 (3), pp.1107-1119. 〈10.1096/fj.201600351R〉. 〈hal-01513615〉

Partager

Métriques

Consultations de la notice

120

Téléchargements de fichiers

36