Intravenous Immunoglobulin Therapy Eliminates Candida albicans and Maintains Intestinal Homeostasis in a Murine Model of Dextran Sulfate Sodium-Induced Colitis

Rogatien Charlet (1, 2, 3) , Boualem Sendid (1, 2, 3) , Srini V. Kaveri (4, 5, 6) , Daniel Poulain (1, 2, 3) , Jagadeesh Bayry (4, 5, 6) , Samir Jawhara (1, 2, 3)

1 LIRIC - Lille Inflammation Research International Center - U 995
2 Faculté de Médecine Henri Warembourg - Université de Lille
3 CHRU Lille - Centre Hospitalier Régional Universitaire [CHU Lille]
4 CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers
5 USPC - Université Sorbonne Paris Cité
6 CRC - Inserm U1138 - Immunopathologie et immunointervention thérapeutique

Intravenous immunoglobulin (IVIg) therapy has diverse anti-inflammatory and immunomodulatory effects and has been employed successfully in autoimmune and inflammatory diseases. The role of IVIg therapy in the modulation of intestinal inflammation and fungal elimination has not been yet investigated. We studied IVIg therapy in a murine model of dextran sulfate sodium (DSS)-induced colitis. Mice received a single oral inoculum of Candida albicans and were exposed to DSS treatment for 2 weeks to induce colitis. All mice received daily IVIg therapy starting on day 1 for 7 days. IVIg therapy not only prevented a loss of body weight caused by the development of colitis but also reduced the severity of intestinal inflammation, as determined by clinical and histological scores. IVIg treatment significantly reduced the Escherichia coli, Enterococcus faecalis, and C. albicans populations in mice. The beneficial effects of IVIg were associated with the suppression of inflammatory cytokine interleukin (IL)-6 and enhancement of IL-10 in the gut. IVIg therapy also led to an increased expression of peroxisome proliferator-activated receptor gamma (PPARγ), while toll-like receptor 4 (TLR-4) expression was reduced. IVIg treatment reduces intestinal inflammation in mice and eliminates C. albicans overgrowth from the gut in association with down-regulation of pro-inflammatory mediators combined with up-regulation of anti-inflammatory cytokines.

Mots clés: intravenous immunoglobulin G; colitis; dextran sulfate sodium; mice; inflammation; cytokines; Candida albicans; Escherichia coli; Enterococcus faecalis

Domaines: Immunothérapie; Maladies infectieuses

Rogatien Charlet, Boualem Sendid, Srini V. Kaveri, Daniel Poulain, Jagadeesh Bayry, et al.. Intravenous Immunoglobulin Therapy Eliminates Candida albicans and Maintains Intestinal Homeostasis in a Murine Model of Dextran Sulfate Sodium-Induced Colitis. International Journal of Molecular Sciences, 2019, 20 (6), pp.1473. ⟨10.3390/ijms20061473⟩. ⟨hal-02122297⟩