Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.

S. Page (1) , F. Vernel-Pauillac (1) , O. O'Connor (1) , S. Bremont (2) , F. Charavay (1) , P. Courvalin (2) , C. Goarant (1) , S. Le Hello (1, 2)

1 Institut Pasteur de Nouvelle-Calédonie
2 Agents antibactériens

Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.

Domains: Life Sciences [q-bio]; Human health and pathology; Infectious diseases

S. Page, F. Vernel-Pauillac, O. O'Connor, S. Bremont, F. Charavay, et al.. Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.. Antimicrobial Agents and Chemotherapy, 2008, 52 (11), pp.4155-8. ⟨10.1128/AAC.00082-08⟩. ⟨pasteur-00451015⟩