Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.

S. Page; F. Vernel-Pauillac; O. O'Connor; S. Bremont; F. Charavay; P. Courvalin; C. Goarant; S. Le Hello

Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.

S. Page ¹ F. Vernel-Pauillac ¹ O. O'Connor ¹ S. Bremont ² F. Charavay ¹ P. Courvalin ² C. Goarant ¹ S. Le Hello ^{1, 2}

1 Institut Pasteur de Nouvelle-Calédonie

Abstract : Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Human health and pathology / Infectious diseases

Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00451015
Contributor : Cyrille Goarant <>
Submitted on : Wednesday, January 27, 2010 - 11:10:27 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM

Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.

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Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.

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