Rapid identification of multidrug-resistant tuberculosis isolates in treatment failure or relapse patients in Bangui, Central African Republic.

F. Minime-Lingoupou (1) , C. Pierre-Audigier (2) , E. Kassa-Kélémbho (1, 2) , N. Barilone (2) , G. Zandanga (1) , J. Rauzier (2) , V. Cadet-Daniel (2) , A. Le Faou (1) , B. Gicquel (2)

1 Institut Pasteur de Bangui
2 Génétique mycobactérienne - Mycobacterial genetics

Multidrug-resistant (MDR) strains were identified in 40% of 54 strains from patients presenting with tuberculosis (TB) treatment failure or relapse in Bangui, Central African Republic. Results obtained with the MTBDRplus line-probe assay or rpoB sequencing were 86% concordant with rifampicin (RMP) resistant phenotypes, while the amplification refractory mutation system test was 71% concordant. No mutation was found in RMP-susceptible strains. MTBDRplus and sequencing were concordant with the detection of the S315T mutation in katG in 95% of MDR strains. Sequencing of pncA suggested pyrazinamide resistance in 50% of MDR strains. Knowledge of these resistances should help to implement treatment in low-income countries.

Domaines: Autre [q-bio.OT]

F. Minime-Lingoupou, C. Pierre-Audigier, E. Kassa-Kélémbho, N. Barilone, G. Zandanga, et al.. Rapid identification of multidrug-resistant tuberculosis isolates in treatment failure or relapse patients in Bangui, Central African Republic.. International Journal of Tuberculosis and Lung Disease, 2010, 14 (6), pp.782-5. ⟨pasteur-00540451⟩