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Journal articles
Formation of virus-like particles from human cell lines exclusively expressing influenza neuraminidase.
Abstract : The minimal virus requirements for the generation of influenza virus-like particle (VLP) assembly and budding were reassessed. Using neuraminidase (NA) from the H5N1 and H1N1 subtypes, it was found that the expression of NA alone was sufficient to generate and release VLPs. Biochemical and functional characterization of the NA-containing VLPs demonstrated that they were morphologically similar to influenza virions. The NA oligomerization was comparable to that of the live virus, and the enzymic activity, whilst not required for the release of NA-VLPs, was preserved. Together, these findings indicate that NA plays a key role in virus budding and morphogenesis, and demonstrate that NA-VLPs represent a useful tool in influenza research.
https://hal-riip.archives-ouvertes.fr/pasteur-00543211
Contributor : Francois Kien Connect in order to contact the contributor
Submitted on : Tuesday, December 6, 2011 - 7:00:07 AM
Last modification on : Monday, October 8, 2018 - 5:44:06 PM
Long-term archiving on: : Wednesday, March 7, 2012 - 2:20:07 AM
Contributor : Francois Kien Connect in order to contact the contributor
Submitted on : Tuesday, December 6, 2011 - 7:00:07 AM
Last modification on : Monday, October 8, 2018 - 5:44:06 PM
Long-term archiving on: : Wednesday, March 7, 2012 - 2:20:07 AM
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Lai_JGenVirol_2010.pdf
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