The SARS coronavirus E protein interacts with PALS1 and alters tight junction formation and epithelial morphogenesis. - RIIP - Réseau International des Instituts Pasteur Accéder directement au contenu
Article Dans Une Revue Molecular Biology of the Cell Année : 2010

The SARS coronavirus E protein interacts with PALS1 and alters tight junction formation and epithelial morphogenesis.

Résumé

Intercellular tight junctions define epithelial apicobasal polarity and form a physical fence which protects underlying tissues from pathogen invasions. PALS1, a tight junction-associated protein, is a member of the CRUMBS3-PALS1-PATJ polarity complex, which is crucial for the establishment and maintenance of epithelial polarity in mammals. Here we report that the carboxy-terminal domain of the SARS-CoV E small envelope protein (E) binds to human PALS1. Using coimmunoprecipitation and pull-down assays, we show that E interacts with PALS1 in mammalian cells and further demonstrate that the last four carboxy-terminal amino acids of E form a novel PDZ-binding motif that binds to PALS1 PDZ domain. PALS1 redistributes to the ERGIC/Golgi region, where E accumulates, in SARS-CoV-infected Vero E6 cells. Ectopic expression of E in MDCKII epithelial cells significantly alters cyst morphogenesis and, furthermore, delays formation of tight junctions, affects polarity, and modifies the subcellular distribution of PALS1, in a PDZ-binding motif-dependent manner. We speculate that hijacking of PALS1 by SARS-CoV E plays a determinant role in the disruption of the lung epithelium in SARS patients.

Domaines

Virologie
Fichier principal
Vignette du fichier
3838.pdf (4.22 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

pasteur-00545808 , version 1 (13-12-2010)

Identifiants

Citer

Kim-Tat Teoh, Yu-Lam Siu, Wing-Lim Chan, Marc A Schlüter, Chia-Jen Liu, et al.. The SARS coronavirus E protein interacts with PALS1 and alters tight junction formation and epithelial morphogenesis.. Molecular Biology of the Cell, 2010, 21 (22), pp.3838-52. ⟨10.1091/mbc.E10-04-0338⟩. ⟨pasteur-00545808⟩

Collections

RIIP RIIP_HONGKONG
177 Consultations
579 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More