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Journal articles
High prevalence and fixation of Plasmodium vivax dhfr/dhps mutations related to sulfadoxine/pyrimethamine resistance in French Guiana.
Céline Barnadas
1
Lise Musset
2, 3
Eric Legrand
3
Magali Tichit
4
Sébastien Briolant
5
Thierry Fusai
5
Christophe Rogier
5
Christiane Bouchier
4
Stéphane Picot
1
Didier Ménard
6
Céline Barnadas 1
Author
Lise Musset 2, 3
Author IdHAL : lise-musset ORCID : https://orcid.org/0000-0003-0215-4110
Eric Legrand 3
Author IdHAL : eric-legrand
Magali Tichit 4
Author
Sébastien Briolant 5
Author
Thierry Fusai 5
Author
Christophe Rogier 5
Author
Christiane Bouchier 4
Author
Stéphane Picot 1
Author
Didier Ménard 6
Author IdHAL : didier-menard ORCID : https://orcid.org/0000-0003-1357-4495
1
Radicaux Libres, Substrats Énergétiques et Physiopathologie Cérébrale (8 Avenue Rockefeller, F-69373 Lyon - France)
- UCBL - Université Claude Bernard Lyon 1 : EA4170 (43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex - France)
- Université de Lyon (92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07 - France)
2
Laboratoire de parasitologie-mycologie EA 209 - Transports membranaires et chimiorésistances (46, rue Henri Huchard 75877 Paris cedex 18 - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- UPD5 - Université Paris Descartes - Paris 5 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
3
CNR - Centre National de Référence du Paludisme [Cayenne, Guyane française] (23 Avenue Pasteur BP 6010 - 97306 Cayenne Cedex - French Guiana)
- Institut Pasteur de la Guyane (23 Avenue Pasteur BP 6010 - 97306 Cayenne Cedex - French Guiana)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
4
Génomique (Plate-Forme) - Genomics Platform (Pôle BIOMICS, CITECH
28 rue du Docteur Roux, 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
5
Unité de Recherche en Biologie et Epidémiologie Parasitaires (UMR6236-IFR48, Institut de Médecine Tropicale du Service de Santé des Armées (IMTSSA), Marseille, France - France)
- Institut de Médecine Tropicale du Service de Santé des Armées (France)
- CNRS - Centre National de la Recherche Scientifique : UMR6236 (France)
6
Institut Pasteur de Madagascar (B.P. 1274 Antananarivo - Madagascar)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
Abstract : Plasmodium vivax isolates from French Guiana were studied for the presence of mutations associated with sulfadoxine/pyrimethamine (SP) drug resistance. Ninety-six blood samples were collected from 2000 to 2005 from symptomatic malaria patients. SP drug resistance was predicted by determining point mutations in the dihydrofolate reductase (pvdhfr) and dihydropteroate synthase (pvdhps) genes. All samples showed mutant genotypes in both genes with a prevalence > 90% for the 58R, 117N, 382C, and 383G. A new mutation (116G) in pvdhfr was found at a frequency of 3.3%. Six different pvdhfr/dhps multilocus genotypes were observed with the predominance of the quintuple mutant-type 58R/117N/173L-382C/383G (59.3%). No significant differences were observed between the prevalence of haplotypes and the year of collection. Our results indicate that, in this area, the fixation of SP drug-resistant parasites in the P. vivax population is stable.
Document type :
Journal articles
Complete list of metadata
https://hal-riip.archives-ouvertes.fr/pasteur-00577267
Contributor : Lise Musset Connect in order to contact the contributor
Submitted on : Wednesday, March 16, 2011 - 11:16:27 PM
Last modification on : Thursday, November 18, 2021 - 3:24:03 PM
Contributor : Lise Musset Connect in order to contact the contributor
Submitted on : Wednesday, March 16, 2011 - 11:16:27 PM
Last modification on : Thursday, November 18, 2021 - 3:24:03 PM
Identifiers
- HAL Id : pasteur-00577267, version 1
- DOI : 10.4269/ajtmh.2009.81.19
- PUBMED : 19556560
Collections
PASTEUR | CNRS | RIIP_GUYANE | RIIP_MADAGASCAR | RIIP | UNIV-PARIS5 | SSA | UNIV-LYON1 | UDL | UNIV-PARIS
Citation
Céline Barnadas, Lise Musset, Eric Legrand, Magali Tichit, Sébastien Briolant, et al.. High prevalence and fixation of Plasmodium vivax dhfr/dhps mutations related to sulfadoxine/pyrimethamine resistance in French Guiana.. American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2009, 81 (1), pp.19-22. ⟨10.4269/ajtmh.2009.81.19⟩. ⟨pasteur-00577267⟩
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