Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum.

Philippe Dussart (1) , Laure Petit (2) , Bhety Labeau (1) , Laetitia Bremand (1) , Alexandre Leduc , David Moua (1) , Séverine Matheus (1) , Laurence Baril (3)

1 Institut Pasteur de la Guyane
2 Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology
3 Institut Pasteur de Dakar

Our findings support the use of diagnostic tools based on the NS1 antigen detection for the diagnosis of acute DENV infection. The immunochromatographic test, Dengue NS1 Ag STRIP-the first rapid diagnostic test for DENV infection-was highly sensitive and specific, and would therefore be a suitable first-line test in the field. The pan-E Dengue Early ELISA was less sensitive than the Platelia test; this two-step ELISA should be combined with DENV IgM antibody detection for the diagnosis of DENV infection.

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Virology

Philippe Dussart, Laure Petit, Bhety Labeau, Laetitia Bremand, Alexandre Leduc, et al.. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum.. PLoS Neglected Tropical Diseases, 2008, 2 (8), pp.e280. ⟨10.1371/journal.pntd.0000280⟩. ⟨pasteur-00584083⟩