Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum.

Philippe Dussart 1, * Bhety Labeau 1 Gisèle Lagathu 2 Philippe Louis 3 Marcio R T Nunes 4 Sueli G. Rodrigues 4 Cécile Storck-Herrmann 5 Raymond Cesaire 2 Jacques Morvan 1 Marie Flamand 6 Laurence Baril 7
* Corresponding author
1 Institut Pasteur de la Guyane
2 Laboratoire de Virologie-Immunologie
3 Laboratoire de Biologie Clinique
4 Department of Arboviruses
5 Laboratoire de Microbiologie
6 Département de Virologie - Department of Virology
7 Epidémiologie des Maladies Emergentes
Abstract : We evaluated a one-step sandwich-format microplate enzyme immunoassay for detecting dengue virus NS1 antigen (Ag) in human serum by use of Platelia Dengue NS1 Ag kits (Bio-Rad Laboratories, Marnes La Coquette, France). We collected 299 serum samples from patients with dengue disease and 50 serum samples from patients not infected with dengue virus. For the 239 serum samples from patients with acute infections testing positive by reverse transcription-PCR and/or virus isolation for one of the four dengue virus serotypes, the sensitivity of the Platelia Dengue NS1 Ag kit was 88.7% (95% confidence interval, 84.0% to 92.4%). None of the serum samples from patients not infected with dengue virus tested positive with the Platelia Dengue NS1 Ag kit. A diagnostic strategy combining the Platelia Dengue NS1 Ag test for acute-phase sera and immunoglobulin M capture enzyme-linked immunosorbent assay for early-convalescent-phase sera increased sensitivity only from 88.7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories.
Document type :
Journal articles
Complete list of metadatas
https://hal-riip.archives-ouvertes.fr/pasteur-00584125
Contributor : Séverine Matheus <>
Submitted on : Thursday, April 7, 2011 - 3:24:30 PM
Last modification on : Thursday, February 7, 2019 - 5:12:54 PM

Links full text

openaccess

Identifiers

Collections

PASTEUR | RIIP | RIIP_GUYANE

Citation

Philippe Dussart, Bhety Labeau, Gisèle Lagathu, Philippe Louis, Marcio R T Nunes, et al.. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum.. Clinical and Vaccine Immunology, American Society for Microbiology, 2006, 13 (11), pp.1185-9. ⟨10.1128/CVI.00229-06⟩. ⟨pasteur-00584125⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

174

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD