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SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway.

Abstract : The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.
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Submitted on : Wednesday, April 27, 2011 - 6:01:56 AM
Last modification on : Wednesday, May 11, 2022 - 5:08:06 PM
Long-term archiving on: : Saturday, December 3, 2016 - 5:40:23 PM

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Choong-Tat Keng, Sara Akerström, Cynthia Sau-Wai Leung, Leo L M Poon, J S Malik Peiris, et al.. SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway.. Microbes and Infection, Elsevier, 2011, 13 (2), pp.179-88. ⟨10.1016/j.micinf.2010.10.017⟩. ⟨pasteur-00588905⟩

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