Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1).

K. H. Chan (1) , S. T. Lai (2) , L. L. M. Poon (1) , Y. Guan (1) , K. Y. Yuen (1) , J. S. M. Peiris (1, 3)

1 Department of Microbiology [HKU]
2 Department of Medicine
3 Centre de recherche Université de Hong-Kong-Pasteur

BACKGROUND: A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic. OBJECTIVES: Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1. STUDY DESIGN: Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods. RESULTS: The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID(50)) log(10)3.3-4.7 was comparable with that of seasonal influenza (TCID(50) log(10)4.0-4.5). CONCLUSION: The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1.

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Virology

K. H. Chan, S. T. Lai, L. L. M. Poon, Y. Guan, K. Y. Yuen, et al.. Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1).. Journal of Clinical Virology, 2009, 45 (3), pp.205-7. ⟨10.1016/j.jcv.2009.05.034⟩. ⟨pasteur-00588942⟩