Evaluation of the intra- and inter-specific genetic variability of Plasmodium lactate dehydrogenase.

Arthur M. Talman 1, 2 Linda Duval 1 Eric Legrand 3 Véronique Hubert 4 Seiha Yen 1 David Bell 5 Jacques Le Bras 6 Frédéric Ariey 1, * Sandrine Houze 6
* Corresponding author
1 Institut Pasteur du Cambodge
2 Division of Cell and Molecular Biology
3 Institut Pasteur de la Guyane
4 Centre National de Référence du Paludisme
5 Malaria and other Vector-borne and parasitic diseases
6 Centre National de Référence du Paludisme
Abstract : BACKGROUND: Malaria diagnosis is vital to efficient control programmes and the recent advent of malaria rapid diagnostic tests (RDTs) provides a reliable and simple diagnostic method. However a characterization of the efficiency of these tests and the proteins they detect is needed to maximize RDT sensitivity. METHODS: Plasmodial lactate dehydrogenase (pLDH) gene of wild isolates of the four human species of Plasmodium from a variety of malaria endemic settings were sequenced and analysed. RESULTS: No variation in nucleotide was found within Plasmodium falciparum, synonymous mutations were found for Plasmodium malariae and Plasmodium. vivax; and three different types of amino acid sequence were found for Plasmodium ovale. Conserved and variable regions were identified within each species. CONCLUSION: The results indicate that antigen variability is unlikely to explain variability in performance of RDTs detecting pLDH from cases of P. falciparum, P. vivax or P. malariae malaria, but may contribute to poor detection of P. ovale.
Document type :
Journal articles
Complete list of metadatas

Cited literature [8 references]  Display  Hide  Download
https://hal-riip.archives-ouvertes.fr/pasteur-00590985
Contributor : Eric Legrand <>
Submitted on : Friday, July 1, 2011 - 3:53:37 PM
Last modification on : Thursday, April 11, 2019 - 4:02:23 PM
Long-term archiving on : Monday, November 12, 2012 - 9:51:24 AM

File

1475-2875-6-140.pdf
Publisher files allowed on an open archive

Identifiers

Collections

RIIP_GUYANE | RIIP | RIIP_CAMBODGE | UNIV-PARIS5 | USPC

Citation

Arthur M. Talman, Linda Duval, Eric Legrand, Véronique Hubert, Seiha Yen, et al.. Evaluation of the intra- and inter-specific genetic variability of Plasmodium lactate dehydrogenase.. Malaria Journal, BioMed Central, 2007, 6, pp.140. ⟨10.1186/1475-2875-6-140⟩. ⟨pasteur-00590985⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

622

Files downloads

377

Contact

CCSD