Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Invasion of Africa by a single pfcrt allele of South East Asian type.

Abstract : BACKGROUND: Because of its dramatic public health impact, Plasmodium falciparum resistance to chloroquine (CQ) has been documented early on. Chloroquine-resistance (CQR) emerged in the late 1950's independently in South East Asia and South America and progressively spread over all malaria areas. CQR was reported in East Africa in the 1970's, and has since invaded the African continent. Many questions remain about the actual selection and spreading process of CQR parasites, and about the evolution of the ancestral mutant gene(s) during spreading. METHODS: Eleven clinical isolates of P. falciparum from Cambodia and 238 from Africa (Senegal, Ivory Coast, Bukina Faso, Mali, Guinea, Togo, Benin, Niger, Congo, Madagascar, Comoros Islands, Tanzania, Kenya, Mozambique, Cameroun, Gabon) were collected during active case detection surveys carried out between 1996 and 2001. Parasite DNA was extracted from frozen blood aliquots and amplification of the gene pfcrt exon 2 (codon 72-76), exon 4 and intron 4 (codon 220 and microsatellite marker) were performed. All fragments were sequenced. RESULTS: 124 isolates with a sensitive (c76/c220:CVMNK/A) haplotype and 125 isolates with a resistant c76/c220:CVIET/S haplotype were found. The microsatellite showed 17 different types in the isolates carrying the c76/c220:CVMNK/A haplotype while all 125 isolates with a CVIET/S haplotype but two had a single microsatellite type, namely (TAAA)3(TA)15, whatever the location or time of collection. CONCLUSION: Those results are consistent with the migration of a single ancestral pfcrt CQR allele from Asia to Africa. This is related to the importance of PFCRT in the fitness of P. falciparum point out this protein as a potential target for developments of new antimalarial drugs.
Document type :
Journal articles
Complete list of metadata

Cited literature [17 references]  Display  Hide  Download
Contributor : Eric Legrand Connect in order to contact the contributor
Submitted on : Thursday, June 30, 2011 - 4:14:22 PM
Last modification on : Thursday, July 7, 2022 - 6:01:40 AM
Long-term archiving on: : Monday, November 12, 2012 - 9:50:21 AM


Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution 4.0 International License



Frédéric Ariey, Thierry Fandeur, Remy Durand, Milijaona Randrianarivelojosia, Ronan Jambou, et al.. Invasion of Africa by a single pfcrt allele of South East Asian type.. Malaria Journal, BioMed Central, 2006, 5, pp.34. ⟨10.1186/1475-2875-5-34⟩. ⟨pasteur-00590988⟩



Record views


Files downloads