 |
Journal articles
Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6.
Abstract : Artemisinin derivatives are an essential component of treatment against multidrug-resistant Plasmodium falciparum malaria. We aimed to investigate in-vitro resistance to artemisinin derivatives in field isolates. In-vitro susceptibility of 530 P falciparum isolates from three countries (Cambodia, French Guiana, and Senegal) with different artemisinin use was assessed with an isotopic microtest. Artemether IC50 up to 117 and 45 nmol/L was seen in French Guiana and Senegal, respectively. DNA sequencing in a subsample of 60 isolates lends support to SERCA-PfATPase6 as the target for artemisinins. The S769N PfATPase6 mutation, noted exclusively in French Guiana, was associated with raised (>30 nmol/L) artemether IC50s (p<0.0001, Mann-Whitney). All resistant isolates came from areas with uncontrolled use of artemisinin derivatives. This rise in resistance indicates the need for increased vigilance and a coordinated and rapid deployment of drug combinations.
https://hal-riip.archives-ouvertes.fr/pasteur-00590989
Contributor : Eric Legrand Connect in order to contact the contributor
Submitted on : Thursday, May 5, 2011 - 11:32:31 PM
Last modification on : Wednesday, January 19, 2022 - 12:48:06 PM
Contributor : Eric Legrand Connect in order to contact the contributor
Submitted on : Thursday, May 5, 2011 - 11:32:31 PM
Last modification on : Wednesday, January 19, 2022 - 12:48:06 PM