 |
 |
Journal articles
Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6.
Ronan Jambou
1, *
Eric Legrand
2
Makhtar Niang
1
Nimol Khim
3
Pharath Lim
3
Béatrice Volney
2
Marie Thérèse Ekala
4
Christiane Bouchier
5
Philippe Esterre
2
Thierry Fandeur
3
Odile Mercereau-Puijalon
4
*
Corresponding author
Ronan Jambou 1
Mail :
Correspondent author
Eric Legrand 2
Mail :
Author IdHAL : eric-legrand
Makhtar Niang 1
Author
Nimol Khim 3
Author
Pharath Lim 3
Author
Béatrice Volney 2
Author
Marie Thérèse Ekala 4
Author
Christiane Bouchier 5
Author
1
Institut Pasteur de Dakar (B.P. 220 Dakar - Senegal)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
2
Institut Pasteur de la Guyane (23 Avenue Pasteur BP 6010 - 97306 Cayenne Cedex - French Guiana)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
3
Institut Pasteur du Cambodge (BP 983 - 5 , Boulevard Monivong, Phnom Penh - Cambodia)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
4
Immunologie moléculaire des parasites (25-28 rue du Docteur Roux F-75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR2581 (France)
5
Génomique (Plate-Forme) (Pôle BIOMICS, CITECH
28 rue du Docteur Roux, 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
Abstract : Artemisinin derivatives are an essential component of treatment against multidrug-resistant Plasmodium falciparum malaria. We aimed to investigate in-vitro resistance to artemisinin derivatives in field isolates. In-vitro susceptibility of 530 P falciparum isolates from three countries (Cambodia, French Guiana, and Senegal) with different artemisinin use was assessed with an isotopic microtest. Artemether IC50 up to 117 and 45 nmol/L was seen in French Guiana and Senegal, respectively. DNA sequencing in a subsample of 60 isolates lends support to SERCA-PfATPase6 as the target for artemisinins. The S769N PfATPase6 mutation, noted exclusively in French Guiana, was associated with raised (>30 nmol/L) artemether IC50s (p<0.0001, Mann-Whitney). All resistant isolates came from areas with uncontrolled use of artemisinin derivatives. This rise in resistance indicates the need for increased vigilance and a coordinated and rapid deployment of drug combinations.
Document type :
Journal articles
Complete list of metadatas
https://hal-riip.archives-ouvertes.fr/pasteur-00590989
Contributor : Eric Legrand <>
Submitted on : Thursday, May 5, 2011 - 11:32:31 PM
Last modification on : Monday, January 13, 2020 - 5:08:06 PM
Contributor : Eric Legrand <>
Submitted on : Thursday, May 5, 2011 - 11:32:31 PM
Last modification on : Monday, January 13, 2020 - 5:08:06 PM
Identifiers
- HAL Id : pasteur-00590989, version 1
- DOI : 10.1016/S0140-6736(05)67787-2
- PUBMED : 16325698
Collections
PASTEUR | CNRS | RIIP_GUYANE | RIIP_DAKAR | RIIP | RIIP_CAMBODGE
Citation
Ronan Jambou, Eric Legrand, Makhtar Niang, Nimol Khim, Pharath Lim, et al.. Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6.. The Lancet, Elsevier, 2005, 366 (9501), pp.1960-3. ⟨10.1016/S0140-6736(05)67787-2⟩. ⟨pasteur-00590989⟩
Metrics
Record views
