Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Replication and packaging of an infectious bursal disease virus segment A-derived minigenome.

Abstract : A synthetic cytomegalovirus (CMV) promoter-driven cDNA minigenome containing the enhanced green fluorescence protein (EGFP) gene as a reporter was derived from the genomic segment A of infectious bursal disease virus (IBDV). The 5'-end of the minigenome was fused to the transcription start site of the immediate early CMV promoter, and the hepatitis delta virus (HDV) ribozyme sequence was added at its 3'-end. We show that co-transfection of the minigenome with a plasmid encoding the IBDV RNA-dependent RNA polymerase VP1, results in a consistent increase of the EGFP expression, as measured by fluorescence microscopy and flow cytometry assays. Replication of the minigenome-derived transcript was evidenced by real-time RT-PCR analyses targeted to both the plus- and minus-sense strands. When cells were infected with IBDV and transfected with the plasmid carrying the minigenome, the minigenome was packaged and EGFP was found to be expressed in a second cycle of infection. These results show the potential use of this system as a new tool to characterize IBDV replication and genome packaging.
Complete list of metadata
Contributor : Dhouieb Najet Connect in order to contact the contributor
Submitted on : Friday, July 1, 2011 - 12:43:56 PM
Last modification on : Saturday, July 3, 2021 - 3:42:54 AM

Links full text



N. Ben Abdeljelil, B. Delmas, H. Mardassi. Replication and packaging of an infectious bursal disease virus segment A-derived minigenome.. Virus Research, Elsevier, 2008, 136 (1-2), pp.146-51. ⟨10.1016/j.virusres.2008.05.002⟩. ⟨pasteur-00605376⟩



Record views