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Journal articles
Purification, synthesis and characterization of AaCtx, the first chlorotoxin-like peptide from Androctonus australis scorpion venom.
Ilhem Rjeibi
1
Kamel Mabrouk
2
Hend Mosrati
1
Caroline Berenguer
3
Hafedh Mejdoub
4
Claude Villard
3
Daniel Laffitte
3
Denis Bertin
2
l'Houcine Ouafik
3
José Luis
3
Mohamed Elayeb
1
Najet Srairi-Abid
1
Ilhem Rjeibi 1
Author
Kamel Mabrouk 2
Author
Hend Mosrati 1
Author
Caroline Berenguer 3
Author IdHAL : daize-berenguer-caroline
Hafedh Mejdoub 4
Author
Claude Villard 3
Author IdHAL : claude-villard ORCID : https://orcid.org/0000-0002-6600-0762
Daniel Laffitte 3
Author
Denis Bertin 2
Author IdHAL : denis-bertin ResearcherId : http://www.researcherid.com/rid/J-9111-2014 IdRef : https://www.idref.fr/06874417X
l'Houcine Ouafik 3
Author IdHAL : l-houcine-ouafik
José Luis 3
Author IdHAL : jose-luis ResearcherId : http://www.researcherid.com/rid/M-3071-2013 ORCID : https://orcid.org/0000-0001-7419-6016
Mohamed Elayeb 1
Author
Najet Srairi-Abid 1
Author
1
Institut Pasteur de Tunis (13, place Pasteur, BP 74, 1002 Tunis Belvédère - Tunisia)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
2
LCP - Laboratoire Chimie Provence (Université de Provence LCP, Equipe Instrumentation et Reactivité Atmosphérique (case 29) 3, place V Hugo 13331 Marseille Cedex 3 - France)
- Université de Provence - Aix-Marseille 1 (3, place Victor Hugo - 13331 Marseille Cedex 03 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR6264 (France)
3
CRO2 - Centre de Recherches en Oncologie biologique et Oncopharmacologie (Faculté de Médecine et Faculté de Pharmacie - 27 Boulevard Jean Moulin 13385 Marseille cedex 05
- France)
- AMU - Aix Marseille Université : U911 (Aix-Marseille Université Jardins du Pharo 58 Boulevard Charles Livon 13284 Marseille cedex 7 - France)
- TIMONE - Hôpital de la Timone [CHU - APHM] : U911 (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U911 (101, rue de Tolbiac, 75013 Paris - France)
4
USCR séquenceur de protéines (route de Soukra, km 3.5, BP 1171, 3000 Sfax - Tunisia)
- Faculté des Sciences de Sfax (Route de la Soukra km 3.5 - B.P. n° 1171 - 3000 Sfax - Tunisia)
- Université de Sfax - University of Sfax (Route de l'Aéroport Km 0.5 - BP 1169 .3029 Sfax - Tunisia)
Abstract : AaCtx is the first chlorotoxin-like peptide isolated from Androctonus australis scorpion venom. Its amino acid sequence shares 70% similarity with chlorotoxin from Leiurus quinquestriatus scorpion venom, from which it differs by twelve amino acids. Due to its very low concentration in venom (0.05%), AaCtx was chemically synthesized. Both native and synthetic AaCtx were active on invasion and migration of human glioma cells. However, their activity was found to be lower than that of chlorotoxin. The molecular model of AaCtx shows that most of amino acids differing between AaCtx and chlorotoxin are localized on the N-terminal loop and the α-helix. Based on known compounds that block chloride channels, we suggest that the absence of negative charged amino acids on AaCtx structure may be responsible for its weak activity on glioma cells migration and invasion. This finding serves as a starting point for structure-function relationship studies leading to design high specific anti-glioma drugs.
Document type :
Journal articles
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https://hal-riip.archives-ouvertes.fr/pasteur-00606677
Contributor : Hadhri Najet <>
Submitted on : Thursday, July 7, 2011 - 9:39:35 AM
Last modification on : Tuesday, June 23, 2020 - 12:30:03 PM
Contributor : Hadhri Najet <>
Submitted on : Thursday, July 7, 2011 - 9:39:35 AM
Last modification on : Tuesday, June 23, 2020 - 12:30:03 PM
Identifiers
- HAL Id : pasteur-00606677, version 1
- DOI : 10.1016/j.peptides.2011.01.015
- PUBMED : 21262299
Collections
CNRS | RIIP | RIIP_TUNIS | UNIV-AMU | INP | PHARMA-AMU
Citation
Ilhem Rjeibi, Kamel Mabrouk, Hend Mosrati, Caroline Berenguer, Hafedh Mejdoub, et al.. Purification, synthesis and characterization of AaCtx, the first chlorotoxin-like peptide from Androctonus australis scorpion venom.. Molecular Ecology, Wiley, 2011, 32 (4), pp.656-63. ⟨10.1016/j.peptides.2011.01.015⟩. ⟨pasteur-00606677⟩
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