Effets immuno-modulateurs des vecteurs d'expressions eucaryotes de l'IL-12 et du GM-CSF en association avec la vaccination à base d'ADN contre la Leishmaniose cutanée expérimentale chez la souris BALB/c

Abstract : Different works of DNA based vaccination against leishmaniasis highlight the complexity of the induced immune responses to fight against the disease. In this work, we exploited the capacity of IL-12 and GMC-SF to activate immune cell mediators and effectors to induce a Th1 response, more capable of clearing the parasite. To generate these immunomodulating activities, we associated eukaryotic expressing vectors of murine IL-12 and GMC-SF to several DNA based vaccine candidates encoding to several L. (L.) major antigens, in the BALB/c mouse. When mice were challenged with a high parasitic load in the hind footpad, no additional protective effect could be generated. However, when the challenge was carried out in the inner face of the ear with a small parasitic load, the association of plasmids encoding to IL-12 and GMC-SF to DNA based vaccination, the protective effects were increased.
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https://hal-riip.archives-ouvertes.fr/pasteur-00612569
Contributor : Institut Pasteur Tunis <>
Submitted on : Friday, July 29, 2011 - 11:50:38 AM
Last modification on : Friday, November 29, 2019 - 11:59:59 AM

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  • HAL Id : pasteur-00612569, version 1
  • PUBMED : 20707219

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S Ben Hadj Ahmed, Koussay Dellagi, C. Bahloul. Effets immuno-modulateurs des vecteurs d'expressions eucaryotes de l'IL-12 et du GM-CSF en association avec la vaccination à base d'ADN contre la Leishmaniose cutanée expérimentale chez la souris BALB/c. Archives de l'Institut Pasteur de Tunis, 2009, 86 (1-4), pp.39-50. ⟨pasteur-00612569⟩

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