Skip to Main content Skip to Navigation
Journal articles

Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.

Abstract : Fourteen ORFs have been identified in the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) genome. ORF 3a of SARS-CoV codes for a recently identified transmembrane protein, but its function remains unknown. In this study we confirmed the 3a protein expression and investigated its localization at the surface of SARS-CoV-infected or 3a-cDNA-transfected cells. Our experiments showed that recombinant 3a protein can form a homotetramer complex through interprotein disulfide bridges in 3a-cDNA-transfected cells, providing a clue to ion channel function. The putative ion channel activity of this protein was assessed in 3a-complement RNA-injected Xenopus oocytes by two-electrode voltage clamp. The results suggest that 3a protein forms a potassium sensitive channel, which can be efficiently inhibited by barium. After FRhK-4 cells were transfected with an siRNA, which is known to suppress 3a expression, followed by infection with SARS-CoV, the released virus was significantly decreased, whereas the replication of the virus in the infected cells was not changed. Our observation suggests that SARS-CoV ORF 3a functions as an ion channel that may promote virus release. This finding will help to explain the highly pathogenic nature of SARS-CoV and to develop new strategies for treatment of SARS infection.
Document type :
Journal articles
Complete list of metadata

Cited literature [26 references]  Display  Hide  Download
Contributor : Xiaojing Lin Connect in order to contact the contributor
Submitted on : Wednesday, September 7, 2011 - 3:41:17 AM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM
Long-term archiving on: : Monday, December 5, 2016 - 12:51:37 AM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Wei Lu, Bo-Jian Zheng, Ke Xu, Wolfgang Schwarz, Lanying Du, et al.. Severe acute respiratory syndrome-associated coronavirus 3a protein forms an ion channel and modulates virus release.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2006, 103 (33), pp.12540-5. ⟨10.1073/pnas.0605402103⟩. ⟨pasteur-00619943⟩



Les métriques sont temporairement indisponibles