Keratinocyte sensitization to tumour necrosis factor-induced nuclear factor kappa B activation by the E2 regulatory protein of human papillomaviruses.

Abstract : Human Papillomavirus life cycle requires extensive manipulation of cell signaling to provide conditions adequate for viral replication within stratified epithelia. In this regard, we show that the E2 regulatory protein of α, β and μ-HPV genotypes enhances TNF-induced activation of NFκB. This activation is mediated by the N-terminal domain of E2, but does not rely on its transcriptional properties. It is independent of the NFκB regulator Tax1BP1, which nevertheless interacts with all the E2 proteins. E2 specifically activates NFκB pathways induced by TNF, while IL1-induced pathways are not affected. E2 stimulates the activating K63-linked ubiquitination of TRAF5, and interacts with both TRAF5 and TRAF6. Our data suggest that E2 potentiates TNF-induced NFκB signaling mediated by TRAF5 activation through direct binding. Since NFκB controls epithelial differentiation, this activity may be involved in commitment of infected keratinocytes to proliferation arrest and differentiation, both required for implementation of the productive viral cycle.
Document type :
Journal articles
Complete list of metadatas

Cited literature [36 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00620950
Contributor : Institut Pasteur Tunis <>
Submitted on : Tuesday, February 26, 2019 - 3:35:11 PM
Last modification on : Tuesday, February 26, 2019 - 3:38:10 PM
Long-term archiving on : Monday, May 27, 2019 - 2:55:46 PM

File

vir.0.032466-0.full-1.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Manel Boulabiar, Samir Boubaker, Michel Favre, Caroline Demeret. Keratinocyte sensitization to tumour necrosis factor-induced nuclear factor kappa B activation by the E2 regulatory protein of human papillomaviruses.. Journal of General Virology, Microbiology Society, 2011, 92 (10), pp.2422-2427. ⟨10.1099/vir.0.032466-0⟩. ⟨pasteur-00620950⟩

Share

Metrics

Record views

241

Files downloads

69