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Luciferase activity was measured 360 20 hours post transfection ( mean of triplicate samples) (b) HaCaT cells were cotransfected with expression plasmid for cherry-Tax1BP1 and GAPDH-directed SiRNA (control) or Tax1BP1-directed Si-RNA. Cherry-Tax1BP1 was detected with antiDsRed Polyclonal antibody, anti-?tubulin was used to normalize for loading. (c) HaCaT cells transfected with p-NF?B-Luc, p-polIII Ren, and GFP (control), GFP-5 E2 or GFP-18 E2 expression plasmids were treated with TNF-? (20 ng/ml) or IL1 (20 ng/ml) before Luciferase activity was measured, GFP-5 E2, GFP-18 E2 or GFP (control) expression plasmids, together with Tax1BP1 Si-RNA or GAPDH Si-RNA (cont) ,
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