Broad humoral and cellular immunity elicited by a bivalent DNA vaccine encoding HA and NP genes from an H5N1 virus.

Ke Xu 1 Zhi-Yang Ling 2 Liang Sun 2 Ying Xu 2 Chao Bian 2 Yuan He 1 Wei Lu 1 Ze Chen 3 Bing Sun 1, 2, *
* Corresponding author
1 LMVI - Key Laboratory of Molecular Virology & Immunology
2 Laboratory of Molecular Cell Biology
3 Shanghai Institute of Biological Products
Abstract : Influenza A virus is highly variable and a major viral respiratory pathogen that can cause severe illness in humans. Therefore it is important to induce a sufficient immune response specific to current strains and to heterosubtypic viruses with vaccines. In this study, we developed a dual-promoter-based bivalent DNA vaccine that encodes both hemagglutinin (HA) and nucleoprotein (NP) proteins from a highly pathogenic A/Chicken/Henan/12/2004 (H5N1) virus. Our results show that the expression levels of HA and NP genes from the dual-promoter plasmid are similar to those seen when they are expressed individually in independent plasmids. When the bivalent DNA vaccine was inoculated via intramuscular injection and in vivo electroporation, high levels of both humoral and cellular immune responses were elicited against homologous H5N1 virus and heterosubtypic H9N2 virus. Furthermore, no obvious antigenic competition was observed between HA and NP proteins in the dual-promoter-based bivalent vaccine compared to monovalent vaccines. Our data suggest that a combination of influenza surface and internal viral genes in a dual-promoter-expressing plasmid may provide a new approach for developing a DNA vaccine that may protect not only specifically against a currently circulating strain, but also may cross-protect broadly against new heterosubtypic viruses.
Document type :
Journal articles
Complete list of metadatas

Cited literature [55 references]  Display  Hide  Download
https://hal-riip.archives-ouvertes.fr/pasteur-00622908
Contributor : Xiaojing Lin <>
Submitted on : Tuesday, September 13, 2011 - 9:12:10 AM
Last modification on : Wednesday, October 17, 2018 - 12:42:06 PM
Long-term archiving on : Wednesday, December 14, 2011 - 2:21:27 AM

File

Viral_Immunology-XKi_SunBi_-20...
Publisher files allowed on an open archive

Identifiers

Collections

RIIP

Citation

Ke Xu, Zhi-Yang Ling, Liang Sun, Ying Xu, Chao Bian, et al.. Broad humoral and cellular immunity elicited by a bivalent DNA vaccine encoding HA and NP genes from an H5N1 virus.. Viral Immunology, Mary Ann Liebert, 2011, 24 (1), pp.45-56. ⟨10.1089/vim.2010.0056⟩. ⟨pasteur-00622908⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

224

Files downloads

236

Contact

CCSD