H. Aihara and J. Miyazaki, Gene transfer into muscle by electroporation in vivo, Nature Biotechnology, vol.3, issue.9, pp.867-870, 1998.
DOI : 10.1016/0378-1119(91)90434-D

S. Babiuk, M. Baca-estrada, M. Foldvari, M. Storms, D. Rabussay et al., Electroporation improves the efficacy of DNA vaccines in large animals, Vaccine, vol.20, issue.27-28, pp.3399-3408, 2002.
DOI : 10.1016/S0264-410X(02)00269-4

C. Bian, X. Zhang, and X. Cai, Conserved amino acids W423 and N424 in receptor-binding domain of SARS-CoV are potential targets for therapeutic monoclonal antibody, Virology, vol.383, issue.1, pp.39-46, 2009.
DOI : 10.1016/j.virol.2008.09.029

K. Butt, G. Smith, and H. Chen, Human Infection with an Avian H9N2 Influenza A Virus in Hong Kong in 2003, Journal of Clinical Microbiology, vol.43, issue.11, pp.5760-5767, 2005.
DOI : 10.1128/JCM.43.11.5760-5767.2005

D. Carragher, D. Kaminski, A. Moquin, L. Hartson, and R. Td, A Novel Role for Non-Neutralizing Antibodies against Nucleoprotein in Facilitating Resistance to Influenza Virus, The Journal of Immunology, vol.181, issue.6, pp.4168-4176, 2008.
DOI : 10.4049/jimmunol.181.6.4168

A. Cauthen, D. Swayne, S. Schultz-cherry, M. Perdue, and D. Suarez, Continued Circulation in China of Highly Pathogenic Avian Influenza Viruses Encoding the Hemagglutinin Gene Associated with the 1997 H5N1 Outbreak in Poultry and Humans, Journal of Virology, vol.74, issue.14, pp.6592-6599, 2000.
DOI : 10.1128/JVI.74.14.6592-6599.2000

D. Centers, . Control, and . Prevention, Swine influenza (H1N1) infection in two children, MMWR Morb Mortal Wkly Rep, vol.58, pp.400-402, 2009.

J. Chen, F. Fang, X. Li, H. Chang, and C. Z. , Protection against influenza virus infection in BALB/c mice immunized with a single dose of neuraminidase-expressing DNAs by electroporation, Vaccine, vol.23, issue.34, pp.4322-4328, 2005.
DOI : 10.1016/j.vaccine.2005.03.035

M. Chen, T. Cheng, and Y. Huang, A consensus-hemagglutinin-based DNA vaccine that protects mice against divergent H5N1 influenza viruses, Proceedings of the National Academy of Sciences, vol.105, issue.36, pp.13538-13543, 2008.
DOI : 10.1073/pnas.0806901105

Z. Chen, S. Kadowaki, Y. Hagiwara, T. Yoshikawa, K. Matsuo et al., Cross-protection against a lethal influenza virus infection by DNA vaccine to neuraminidase, Vaccine, vol.18, issue.28, pp.3214-3222, 2000.
DOI : 10.1016/S0264-410X(00)00149-3

Z. Chen, P. Zhou, D. Ho, N. Landau, and P. Marx, Genetically divergent strains of simian immunodeficiency virus use CCR5 as a coreceptor for entry, J Virol, vol.71, pp.2705-2714, 1997.

E. Claas, Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus, The Lancet, vol.351, issue.9101, pp.472-477, 1998.
DOI : 10.1016/S0140-6736(97)11212-0

R. Coles, S. Mueller, W. Heath, F. Carbone, and A. Brooks, Progression of Armed CTL from Draining Lymph Node to Spleen Shortly After Localized Infection with Herpes Simplex Virus 1, The Journal of Immunology, vol.168, issue.2, pp.834-838, 2002.
DOI : 10.4049/jimmunol.168.2.834

R. Couch, An overview of serum antibody responses to influenza virus antigens, Dev Biol (Basel), vol.115, pp.25-30, 2003.

H. Davis, Intramuscular and intradermal injection of DNA vaccine in mice and primates methods in molecular medicine, DNA Vaccine Methods Protocols, vol.29

D. Jong, J. Claas, E. Osterhaus, A. Webster, R. Lim et al., A pandemic warning?, Nature, vol.389, issue.6651, p.554, 1997.
DOI : 10.1038/39218

Y. Deng, J. Yewdell, L. Eisenlohr, and J. Bennink, MHC affinity, peptide liberation, T cell repertoire, and immunodominance all contribute to the paucity of MHC class I-restricted peptides recognized by antiviral CTL, J Immunol, vol.158, pp.1507-1515, 1997.

L. Dimenna and H. Ertl, Pandemic Influenza Vaccines, Curr Top Microbiol Immunol, vol.333, pp.291-321, 2009.
DOI : 10.1007/978-3-540-92165-3_15

J. Donnelly, A. Friedman, and D. Martinez, Preclinical efficacy of a prototype DNA vaccine: Enhanced protection against antigenic drift in influenza virus, Nature Medicine, vol.12, issue.6, pp.583-587, 1995.
DOI : 10.1038/nm0695-583

J. Donnelly, A. Friedman, J. Ulmer, and M. Liu, Further protection against antigenic drift of influenza virus in a ferret model by DNA vaccination, Vaccine, vol.15, issue.8, pp.865-868, 1997.
DOI : 10.1016/S0264-410X(96)00268-X

J. Donnelly, J. Ulmer, J. Shiver, and M. Liu, DNA VACCINES, Annual Review of Immunology, vol.15, issue.1, pp.617-648, 1997.
DOI : 10.1146/annurev.immunol.15.1.617

S. Epstein, T. Tumpey, and J. Misplon, DNA Vaccine Expressing Conserved Influenza Virus Proteins Protective Against H5N1 Challenge Infection in Mice, Emerging Infectious Diseases, vol.8, issue.8, pp.796-801, 2002.
DOI : 10.3201/eid0805.010476

C. Fraser, C. Donnelly, and S. Cauchemez, Pandemic Potential of a Strain of Influenza A (H1N1): Early Findings, Science, vol.324, issue.5934, pp.1557-1561, 2009.
DOI : 10.1126/science.1176062

T. Fu, J. Ulmer, and M. Caulfield, Priming of cytotoxic T lymphocytes by DNA vaccines: requirement for professional antigen presenting cells and evidence for antigen transfer from myocytes, Mol Med, vol.3, pp.362-371, 1997.

R. Garten, C. Davis, and C. Russell, Antigenic and Genetic Characteristics of Swine-Origin 2009 A(H1N1) Influenza Viruses Circulating in Humans, Science, vol.325, issue.5937, pp.197-201, 2009.
DOI : 10.1126/science.1176225

W. Gerhard, K. Mozdzanowska, and D. Zharikova, Prospects for Universal Influenza Virus Vaccine, Emerging Infectious Diseases, vol.12, issue.4, pp.569-574, 2006.
DOI : 10.3201/eid1204.051020

H. Jin, C. Xiao, and Z. Chen, Induction of Th1 type response by DNA vaccinations with N, M, and E genes against SARS-CoV in mice, Biochemical and Biophysical Research Communications, vol.328, issue.4, pp.979-986, 2005.
DOI : 10.1016/j.bbrc.2005.01.048

B. Johansson and E. Kilbourne, Immunization with Dissociated Neuraminidase, Matrix, and Nucleoproteins from Influenza A Virus Eliminates Cognate Help and Antigenic Competition, Virology, vol.225, issue.1, pp.136-144, 1996.
DOI : 10.1006/viro.1996.0581

S. Kodihalli, H. Goto, D. Kobasa, S. Krauss, Y. Kawaoka et al., DNA vaccine encoding hemagglutinin provides protective immunity against H5N1 influenza virus infection in mice, J Virol, vol.73, pp.2094-2098, 1999.

S. Kodihalli, J. Haynes, H. Robinson, and R. Webster, Cross-protection among lethal H5N2 influenza viruses induced by DNA vaccine to the hemagglutinin, J Virol, vol.71, pp.3391-3396, 1997.

Y. Lin, M. Shaw, and V. Gregory, Avian-to-human transmission of H9N2 subtype influenza A viruses: Relationship between H9N2 and H5N1 human isolates, Proceedings of the National Academy of Sciences, vol.97, issue.17, pp.9654-9658, 2000.
DOI : 10.1073/pnas.160270697

I. Nefkens, J. Garcia, and C. Ling, Hemagglutinin pseudotyped lentiviral particles: Characterization of a new method for avian H5N1 influenza sero-diagnosis, Journal of Clinical Virology, vol.39, issue.1, pp.27-33, 2007.
DOI : 10.1016/j.jcv.2007.02.005

URL : https://hal.archives-ouvertes.fr/pasteur-00589029

S. Oehen and K. Brduscha-riem, Differentiation of naive CTL to effector and memory CTL: correlation of effector function with phenotype and cell division, J Immunol, vol.161, pp.5338-5346, 1998.

P. Palese and A. Garcia-sastre, Influenza vaccines: present and future, Journal of Clinical Investigation, vol.110, issue.1, pp.9-13, 2002.
DOI : 10.1172/JCI0215999

E. Prokudina and N. Semenova, Localization of the influenza virus nucleoprotein: cell-associated and extracellular non-virion forms, Journal of General Virology, vol.72, issue.7, pp.1699-1702, 1991.
DOI : 10.1099/0022-1317-72-7-1699

S. Riedel, Crossing the species barrier: the threat of an avian influenza pandemic, ProcBayl Univ Med Cent), vol.19, pp.16-20, 2006.

H. Robinson, L. Hunt, and R. Webster, Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA, Vaccine, vol.11, issue.9, pp.957-960, 1993.
DOI : 10.1016/0264-410X(93)90385-B

J. Scheerlinck, J. Karlis, T. Tjelle, P. Presidente, I. Mathiesen et al., In vivo electroporation improves immune responses to DNA vaccination in sheep, Vaccine, vol.22, issue.13-14, pp.1820-1825, 2004.
DOI : 10.1016/j.vaccine.2003.09.053

L. Stitz, C. Schmitz, D. Binder, R. Zinkernagel, E. Paoletti et al., Characterization and Immunological Properties of Influenza A Virus Nucleoprotein (NP): Cell-associated NP Isolated from Infected Cells or Viral NP Expressed by Vaccinia Recombinant Virus do not Confer Protection, Journal of General Virology, vol.71, issue.5, pp.1169-1179, 1990.
DOI : 10.1099/0022-1317-71-5-1169

K. Subbarao, Characterization of an Avian Influenza A (H5N1) Virus Isolated from a Child with a Fatal Respiratory Illness, Science, vol.279, issue.5349, pp.393-396, 1998.
DOI : 10.1126/science.279.5349.393

K. Subbarao, H. Chen, and D. Swayne, Evaluation of a Genetically Modified Reassortant H5N1 Influenza A Virus Vaccine Candidate Generated by Plasmid-Based Reverse Genetics, Virology, vol.305, issue.1, pp.192-200, 2003.
DOI : 10.1006/viro.2002.1742

S. Tollefsen, T. Tjelle, and J. Schneider, Improved cellular and humoral immune responses against Mycobacterium tuberculosis antigens after intramuscular DNA immunisation combined with muscle electroporation, Vaccine, vol.20, issue.27-28, pp.3370-3378, 2002.
DOI : 10.1016/S0264-410X(02)00289-X

C. Tsai, C. Caillet, and H. Hu, Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase, Vaccine, vol.27, issue.48, pp.6777-6790, 2009.
DOI : 10.1016/j.vaccine.2009.08.056

URL : https://hal.archives-ouvertes.fr/pasteur-00625272

J. Ulmer, Influenza DNA vaccines, Vaccine, vol.20, issue.2, pp.74-76, 2002.
DOI : 10.1016/S0264-410X(02)00136-6

J. Ulmer, R. Deck, C. Dewitt, A. Friedman, J. Donnelly et al., Protective immunity by intramuscular injection of low doses of influenza virus DNA vaccines, Vaccine, vol.12, issue.16, pp.1541-1544, 1994.
DOI : 10.1016/0264-410X(94)90081-7

J. Ulmer, J. Donnelly, and S. Parker, Heterologous protection against influenza by injection of DNA encoding a viral protein, Science, vol.259, issue.5102, pp.1745-1749, 1993.
DOI : 10.1126/science.8456302

J. Ulmer, T. Fu, and R. Deck, Protective CD4þ and CD8þ T cells against influenza virus induced by vaccination with nucleoprotein DNA, J Virol, vol.72, pp.5648-5653, 1998.

J. Virelizier, A. Allison, J. Oxford, and G. Schild, Early presence of ribonucleoprotein antigen on surface of influenza virus-infected cells, Nature, vol.39, issue.5597, pp.52-54, 1977.
DOI : 10.1038/256613a0

D. Wells, Gene Therapy Progress and Prospects: Electroporation and other physical methods, Gene Therapy, vol.11, issue.18, pp.1363-1369, 2004.
DOI : 10.1038/sj.gt.3302337

G. Widera, M. Austin, and D. Rabussay, Increased DNA Vaccine Delivery and Immunogenicity by Electroporation In Vivo, The Journal of Immunology, vol.164, issue.9, pp.4635-4640, 2000.
DOI : 10.4049/jimmunol.164.9.4635

S. Wong and K. Yuen, Avian Influenza Virus Infections in Humans, Chest, vol.129, issue.1, pp.156-168, 2006.
DOI : 10.1378/chest.129.1.156

J. Wood and J. Robertson, From lethal virus to life-saving vaccine: developing inactivated vaccines for pandemic influenza, Nature Reviews Microbiology, vol.19, issue.10, pp.842-847, 2004.
DOI : 10.1038/nrmicro979

Z. Yang, C. Wei, W. Kong, L. Wu, L. Xu et al., Immunization by Avian H5 Influenza Hemagglutinin Mutants with Altered Receptor Binding Specificity, Science, vol.317, issue.5839, pp.825-828, 2007.
DOI : 10.1126/science.1135165

J. Yewdell, J. Bennink, G. Smith, and M. B. , Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes., Proceedings of the National Academy of Sciences, vol.82, issue.6, pp.1785-1789, 1985.
DOI : 10.1073/pnas.82.6.1785

L. Zheng, F. Wang, Z. Yang, J. Chen, H. Chang et al., A single immunization with HA DNA vaccine by electroporation induces early protection against H5N1 avian influenza virus challenge in mice, BMC Infectious Diseases, vol.26, issue.50, pp.17-56, 2009.
DOI : 10.1016/j.vaccine.2008.08.046