Genetic diversity and quinolone resistance in Campylobacter jejuni isolates from poultry in Senegal.

Alfred Dieudonné Kinana 1, * Eric Cardinale 2 Fatou Tall 3 Ibrahim Bahsoun 1 Jean-Marie Sire 1 Benoit Garin 1 Sebastien Breurec 1 Cheikh Saad-Bouh Boye 4 Jean-David Perrier-Gros-Claude 5
* Corresponding author
1 Bactériologie médicale et Environnementale
2 Cirad-EMVT PPA - Programme système de Production Animale
EMVT - Département Elevage et médecine vétérinaire
3 LNERV - Laboratoire National d'Elevage et de Recherches Vétérinaires [Dakar]
4 Laboratoire de Bactériologie-Virologie
5 Institut Pasteur du Maroc
Abstract : We used the multilocus sequence typing (MLST) method to evaluate the genetic diversity of 46 Campylobacter jejuni isolates from chickens and to determine the link between quinolone resistance and sequence type (ST). There were a total of 16 ST genotypes, and the majority of them belonged to seven clonal complexes previously identified by using isolates from human disease. The ST-353 complex was the most common complex, whereas the ST-21, ST-42, ST-52, and ST-257 complexes were less well represented. The resistance phenotype varied for each ST, and the Thr-86-Ile substitution in the GyrA protein was the predominant mechanism of resistance to quinolone. Nine of the 14 isolates having the Thr-86-Ile substitution belonged to the ST-353 complex. MLST showed that the emergence of quinolone resistance is not related to the diffusion of a unique clone and that there is no link between ST genotype and quinolone resistance. Based on silent mutations, different variants of the gyrA gene were shown to exist for the same ST. These data provide useful information for understanding the epidemiology of C. jejuni in Senegal.
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Journal articles
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Submitted on : Friday, September 16, 2011 - 6:07:45 PM
Last modification on : Monday, January 20, 2020 - 11:56:10 AM

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Alfred Dieudonné Kinana, Eric Cardinale, Fatou Tall, Ibrahim Bahsoun, Jean-Marie Sire, et al.. Genetic diversity and quinolone resistance in Campylobacter jejuni isolates from poultry in Senegal.. Applied and Environmental Microbiology, American Society for Microbiology, 2006, 72 (5), pp.3309-13. ⟨10.1128/AEM.72.5.3309-3313.2006⟩. ⟨pasteur-00624407⟩

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