Influenza virus genome C4 promoter/origin attenuates its transcription and replication activity by the low polymerase recognition activity.

Abstract : A natural variation is observed at position 4 of the 3'-end of influenza A virus genomes, where U (U4) or C (C4) is present. The replicon activity of C4 was 28% of U4. We compared the transcription and replication activity of U4 [v84(U4)], C4 [v84(C4)] and the complimentary RNA (c84) using the purified influenza virus RNA polymerase in vitro. ApG-primed replication activities of v84(C4) and c84 were 23.8% and 7.8% of v84(U4). Globin mRNA-primed transcription activities of v84(C4) and c84 were 36.9% and 6.81% of v84(U4). De novo replication activities of v84(C4) and c84 were 21.3 and 10.2% of v84(U4). This difference came from their polymerase binding activity. When all the eight genome segments of WSN strain were changed to U4, the virus titer was 760 times higher than the wild type. However, its pathogenicity in mice was lower than the wild type.
Document type :
Journal articles
Complete list of metadatas

Cited literature [44 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00624959
Contributor : Xiaojing Lin <>
Submitted on : Tuesday, September 20, 2011 - 11:24:40 AM
Last modification on : Wednesday, October 17, 2018 - 12:42:06 PM
Long-term archiving on: Sunday, December 4, 2016 - 5:58:31 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Hongbing Jiang, Shijian Zhang, Qiang Wang, Jinlan Wang, Liqing Geng, et al.. Influenza virus genome C4 promoter/origin attenuates its transcription and replication activity by the low polymerase recognition activity.. Virology, Elsevier, 2010, 408 (2), pp.190-6. ⟨10.1016/j.virol.2010.09.022⟩. ⟨pasteur-00624959⟩

Share

Metrics

Record views

104