Correlation between bocavirus infection and humoral response, and co-infection with other respiratory viruses in children with acute respiratory infection.

Abstract : BACKGROUND: Human bocavirus (HBoV), a recently discovered virus, is prevalent among children with respiratory tract infection throughout the world. Co-infection was frequently found in HBoV-positive patients. Thus, whether HBoV is responsible for the respiratory disease is still arguable. OBJECTIVES: A comprehensive study was carried out to integrate clinical and virological prevalence in HBoV-positive outpatient children, and to determine genetic and serologic characteristics of HBoV in Shanghai, China. STUDY DESIGN: Nasal/throat swabs and sera were obtained over a 2-year period from 817 children with respiratory tract infection to examine the presence of HBoV and its co-infection. The seroepidemiology of HBoV was studied by ELISA and Western blot against the capsid protein VP2-based fragment. Persistence of HBoV was also analyzed in 12 pairs of return-visit cases. RESULTS: HBoV was identified in 96 samples (11.8%). The co-infection rate with other respiratory viruses was 51%. IgM was detected in 55.7% of HBoV RT-PCR-positive patients, and in 72.7% of those who had high viral genome load. In addition, persistent viral DNA positivity was detected in 10 of 12 HBoV-positive cases tested, an average of 14 days later, and one child was still HBoV-positive after 31 days. CONCLUSION: HBoV was found frequently in children with respiratory tract symptoms associated with other respiratory viruses, and persisted in the respiratory tract and in serum and urine. The presence of IgM was significantly more prevalent in viremic patients and those diagnosed with high load of HBoV DNA in nasal/throat swabs.
Document type :
Journal articles
Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00625311
Contributor : Xiaojing Lin <>
Submitted on : Wednesday, September 21, 2011 - 12:02:57 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM
Long-term archiving on : Thursday, March 30, 2017 - 3:29:29 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Kai Wang, Wei Wang, Huajie Yan, Peijun Ren, Jing Zhang, et al.. Correlation between bocavirus infection and humoral response, and co-infection with other respiratory viruses in children with acute respiratory infection.. J Clin Virol, 2010, 47 (2), pp.148-55. ⟨10.1016/j.jcv.2009.11.015⟩. ⟨pasteur-00625311⟩

Share

Metrics

Record views

123