Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods.

John M. Nicholls; Linda P. W. Wong; Renee W. Y. Chan; Leo L. M. Poon; Leo K. Y. So; Hui-Ling Yen; Kevin Fung; Sjouke van Poucke; J. S. Malik Peiris

Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods.

John M. Nicholls ^{1, *} Linda P. W. Wong ^{1, 2} Renee W. Y. Chan ² Leo L. M. Poon ² Leo K. Y. So ² Hui-Ling Yen ³ Kevin Fung ¹ Sjouke van Poucke ⁴ J. S. Malik Peiris ²

* Corresponding author

1 Department of pathology [HKU]

2 Department of Microbiology [HKU]

3 HKU - The University of Hong Kong

4 Laboratory of Virology

Abstract : Tissues infected with highly pathogenic avian influenza viruses such as H5N1 and H7N7 are normally required to be fixed in formalin or paraformaldehyde before examination in order to inactivate the virus. In this study commercially available monoclonal antibodies to the influenza nucleoprotein (NP) were evaluated in order to determine which antibodies would identify positive cells in tissues fixed in formalin or paraformaldehyde. An assessment of which antigen retrieval process would unmask antigens blocked by formalin fixation was also made. Of six commercially available monoclonal antibodies tested, only one (HB65, European Veterinary Laboratories) was able to identify all formalin fixed avian, swine and human influenza virus infected tissues, and this was after pronase induced epitope retrieval. This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde.

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology / Virology

Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00644726
Contributor : Francois Kien <>
Submitted on : Friday, November 25, 2011 - 4:25:12 AM
Last modification on : Tuesday, November 29, 2011 - 4:08:17 PM

Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods.

Identifiers

Collections

Citation

Export

Metrics

115

Contact

Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods.

Identifiers

Collections

Citation

Export

Share

Metrics

115

Contact