Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex. - Archive ouverte HAL Access content directly
Journal Articles Chemical Communications Year : 2010

Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex.

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Abstract

A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.

Dates and versions

pasteur-00683850 , version 1 (03-04-2012)

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Youngseon Choi, Junghan Lee, Keumhyun Kim, Heeyeon Kim, Peter Sommer, et al.. Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex.. Chemical Communications, 2010, 46 (48), pp.9146-8. ⟨10.1039/c0cc02702b⟩. ⟨pasteur-00683850⟩

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