Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex.

Youngseon Choi (1) , Junghan Lee (1) , Keumhyun Kim (1) , Heeyeon Kim (1) , Peter Sommer (1) , Rita Song (1)

1 Institut Pasteur Korea - Institut Pasteur de Corée

A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.

Domaines: Virologie

Youngseon Choi, Junghan Lee, Keumhyun Kim, Heeyeon Kim, Peter Sommer, et al.. Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex.. Chemical Communications, 2010, 46 (48), pp.9146-8. ⟨10.1039/c0cc02702b⟩. ⟨pasteur-00683850⟩