Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

The eisosome core is composed of BAR domain proteins.

Abstract : Eisosomes define sites of plasma membrane organization. In Saccharomyces cerevisiae, eisosomes delimit furrow-like plasma membrane invaginations that concentrate sterols, transporters, and signaling molecules. Eisosomes are static macromolecular assemblies composed of cytoplasmic proteins, most of which have no known function. In this study, we used a bioinformatics approach to analyze a set of 20 eisosome proteins. We found that the core components of eisosomes, paralogue proteins Pil1 and Lsp1, are distant homologues of membrane-sculpting Bin/amphiphysin/Rvs (BAR) proteins. Consistent with this finding, purified recombinant Pil1 and Lsp1 tubulated liposomes and formed tubules when the proteins were overexpressed in mammalian cells. Structural homology modeling and site-directed mutagenesis indicate that Pil1 positively charged surface patches are needed for membrane binding and liposome tubulation. Pil1 BAR domain mutants were defective in both eisosome assembly and plasma membrane domain organization. In addition, we found that eisosome-associated proteins Slm1 and Slm2 have F-BAR domains and that these domains are needed for targeting to furrow-like plasma membrane invaginations. Our results support a model in which BAR domain protein-mediated membrane bending leads to clustering of lipids and proteins within the plasma membrane.
Document type :
Journal articles
Complete list of metadata

Cited literature [61 references]  Display  Hide  Download
Contributor : Mariella Botta Connect in order to contact the contributor
Submitted on : Thursday, April 26, 2012 - 2:09:08 PM
Last modification on : Thursday, March 17, 2022 - 12:20:04 PM
Long-term archiving on: : Wednesday, December 14, 2016 - 7:49:33 PM


Publisher files allowed on an open archive




Agustina Olivera-Couto, Martin Graña, Laura Harispe, Pablo S Aguilar. The eisosome core is composed of BAR domain proteins.. Molecular Biology of the Cell, American Society for Cell Biology, 2011, 22 (13), pp.2360-72. ⟨10.1091/mbc.E10-12-1021⟩. ⟨pasteur-00685065⟩



Record views


Files downloads