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Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate.

Abstract : An N-terminally truncated version of the enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi lacking the first 37 residues was crystallized both in its apo form and in a binary complex with glucose 6-phosphate. The crystals both belonged to space group P2(1) and diffracted to 2.85 and 3.35 Å resolution, respectively. Self-rotation function maps were consistent with point group 222. The structure was solved by molecular replacement, confirming a tetrameric quaternary structure.
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Cecilia Ortíz, Nicole Larrieux, Andrea Medeiros, Horacio Botti, Marcelo Comini, et al.. Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate.. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 2011, 67 (Pt 11), pp.1457-61. ⟨10.1107/S1744309111037821⟩. ⟨pasteur-00686320⟩

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