Cloning, localization and differential expression of the Trypanosoma cruzi TcOGNT-2 glycosyl transferase.

María Laura Chiribao; María Gabriela Libisch; Eduardo Osinaga; Adriana Parodi-Talice; Carlos Robello

Cloning, localization and differential expression of the Trypanosoma cruzi TcOGNT-2 glycosyl transferase.

María Laura Chiribao ^{1, 2} María Gabriela Libisch ¹ Eduardo Osinaga ³ Adriana Parodi-Talice ^{1, 4} Carlos Robello ^{1, 2, *}

* Corresponding author

1 Institut Pasteur de Montevideo

2 Departamento de Bioquimica

3 Departamento de inmunobiologia

4 Seccion Genetica

Abstract : The surface of Trypanosoma cruzi is covered by a dense glycocalix which is characteristic of each stage of the life cycle. Its composition and complexity depend mainly on mucin-like proteins. A remarkable feature of O-glycan biosynthesis in trypanosomes is that it initiates with the addition of a GlcNAc instead of the GalNAc residue that is commonly used in vertebrate mucins. The fact that the interplay between trans-sialidase and mucin is crucial for pathogenesis, and both families have stage-specific members is also remarkable. Recently the enzyme that transfers the first GlcNAc from UDP-GlcNAc to a serine or threonine residue was kinetically characterized. The relevance of this enzyme is evidenced by its role as catalyzer of the first step in O-glycosylation. In this paper we describe how this gene is expressed differentially along the life cycle with a pattern that is very similar to that of trans-sialidases. Its localization was determined, showing that the protein predicted to be in the Golgi apparatus is also present in reservosomes. Finally our results indicate that this enzyme, when overexpressed, enhances T. cruzi infectivity.

Keywords : Chagas disease Glycosyl-transferase Differential expression O-glycosylation Infectivity

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology

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https://hal-riip.archives-ouvertes.fr/pasteur-00686579
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Submitted on : Tuesday, April 10, 2012 - 4:00:45 PM
Last modification on : Friday, February 22, 2019 - 11:16:45 AM

Cloning, localization and differential expression of the Trypanosoma cruzi TcOGNT-2 glycosyl transferase.

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Cloning, localization and differential expression of the Trypanosoma cruzi TcOGNT-2 glycosyl transferase.

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