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TB-Lineage: An online tool for classification and analysis of strains of Mycobacterium tuberculosis complex.

Abstract : This paper formulates a set of rules to classify genotypes of the Mycobacterium tuberculosis complex (MTBC) into major lineages using spoligotypes and MIRU-VNTR results. The rules synthesize prior literature that characterizes lineages by spacer deletions and variations in the number of repeats seen at locus MIRU24 (alias VNTR2687). A tool that efficiently and accurately implements this rule base is now freely available at http://tbinsight.cs.rpi.edu/run_tb_lineage.html. When MIRU24 data is not available, the system utilizes predictions made by a Naïve Bayes classifier based on spoligotype data. This website also provides a tool to generate spoligoforests in order to visualize the genetic diversity and relatedness of genotypes and their associated lineages. A detailed analysis of the application of these tools on a dataset collected by the CDC consisting of 3198 distinct spoligotypes and 5430 distinct MIRU-VNTR types from 37,066 clinical isolates is presented. The tools were also tested on four other independent datasets. The accuracy of automated classification using both spoligotypes and MIRU24 is >99%, and using spoligotypes alone is >95%. This online rule-based classification technique in conjunction with genotype visualization provides a practical tool that supports surveillance of TB transmission trends and molecular epidemiological studies.
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https://hal-riip.archives-ouvertes.fr/pasteur-00691749
Contributor : Nalin Rastogi <>
Submitted on : Thursday, April 26, 2012 - 11:38:11 PM
Last modification on : Tuesday, February 12, 2019 - 4:10:06 PM

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Amina Shabbeer, Lauren S Cowan, Cagri Ozcaglar, Nalin Rastogi, Scott L Vandenberg, et al.. TB-Lineage: An online tool for classification and analysis of strains of Mycobacterium tuberculosis complex.. Infection, Genetics and Evolution, Elsevier, 2012, 12 (4), pp.789-97. ⟨10.1016/j.meegid.2012.02.010⟩. ⟨pasteur-00691749⟩

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