Skip to Main content Skip to Navigation
Journal articles

Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic diversity of Mycobacterium tuberculosis in Delhi, North India.

Abstract : The aim of the present study was to compare polymerase chain reaction (PCR)-based methods--spoligotyping and mycobacterial interspersed repetitive units (MIRU) typing--with the gold-standard IS6110 restriction fragment length polymorphism (RFLP) analysis in 101 isolates of Mycobacterium tuberculosis to determine the genetic diversity of M. tuberculosis clinical isolates from Delhi, North India. Spoligotyping resulted in 49 patterns (14 clusters); the largest cluster was composed of Spoligotype International Types (SITs)26 [Central-Asian (CAS)1-Delhi lineage], followed by SIT11 [East-African-Indian (EAI) 3-Indian lineage]. A large number of isolates (75%) belonged to genotypic lineages, such as CAS, EAI and Manu, with a high specificity for the Indian subcontinent, emphasising the complex diversity of the phylogenetically coherent M. tuberculosis in North India. MIRU typing, using 11 discriminatory loci, was able to distinguish between all but two strains based on individual patterns. IS6110-RFLP analysis (n = 80 strains) resulted in 67 unique isolates and four clusters containing 13 strains. MIRUs discriminated all 13 strains, whereas spoligotyping discriminated 11 strains. Our results validate the use of PCR-based molecular typing of M. tuberculosis using repetitive elements in Indian isolates and demonstrate the usefulness of MIRUs for discriminating low-IS6110-copy isolates, which accounted for more than one-fifth of the strains in the present study.
Document type :
Journal articles
Complete list of metadatas

Cited literature [38 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00691755
Contributor : Nalin Rastogi <>
Submitted on : Tuesday, May 15, 2012 - 10:46:38 AM
Last modification on : Wednesday, October 30, 2019 - 2:26:09 PM
Long-term archiving on: : Thursday, August 16, 2012 - 2:20:25 AM

File

comparison_of_spoligotyping.pd...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Mandira Varma-Basil, Sujeet Kumar, Jyoti Arora, Archana Angrup, Thierry Zozio, et al.. Comparison of spoligotyping, mycobacterial interspersed repetitive units typing and IS6110-RFLP in a study of genotypic diversity of Mycobacterium tuberculosis in Delhi, North India.. Memórias do Instituto Oswaldo Cruz., Instituto Oswaldo Cruz, Ministério da Saúde, 2011, 106 (5), pp.524-535. ⟨10.1590/S0074-02762011000500002⟩. ⟨pasteur-00691755⟩

Share

Metrics

Record views

285

Files downloads

277