Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil.

Abstract : We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region.
Document type :
Journal articles
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00691759
Contributor : Nalin Rastogi <>
Submitted on : Tuesday, May 15, 2012 - 10:50:15 AM
Last modification on : Monday, October 8, 2018 - 5:44:05 PM
Long-term archiving on : Thursday, August 16, 2012 - 2:20:34 AM

File

spoligotyping_of_clinical_myco...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Silvana Spíndola De Miranda, Wânia da Silva Carvalho, Philip Noel Suffys, Afrânio Lineu Kritski, Martha Oliveira, et al.. Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil.. Memórias do Instituto Oswaldo Cruz., Instituto Oswaldo Cruz, Ministério da Saúde, 2011, 106 (3), pp.267-73. ⟨10.1590/S0074-02762011000300003⟩. ⟨pasteur-00691759⟩

Share

Metrics

Record views

254

Files downloads

392