Penitentiary population of Mycobacterium tuberculosis in Kyrgyzstan: exceptionally high prevalence of the Beijing genotype and its Russia-specific subtype.

Igor Mokrousov (1, 2) , Violeta Valcheva (1, 3) , Nurmira Sovhozova (4) , Almaz Aldashev (4) , Nalin Rastogi (1) , Jainagul Isakova (4)

1 Institut Pasteur de la Guadeloupe
2 Institut Pasteur de Saint-Pétersbourg
3 Institut Stephan Angeloff
4 Institute of Molecular Biology and Medicine

Here, we present results of the first study of the Mycobacterium tuberculosis genotypes circulating in Kyrgyzstan. We focused on the incarcerated population known to be at high-risk for tuberculosis (TB) and with a significant impact on TB incidence in the general population. Beijing genotype was detected in 42 of 56 M. tuberculosis sputum-extracted DNA samples from newly-diagnosed adult pulmonary TB patients. RIF and INH resistance was genotypically detected in 28% and 55% samples; 13 of 15 MDR strains belonged to Beijing genotype. 12-locus MIRU-VNTR typing showed 8 of 56 samples to be mixed cases; 7 of them contained a Beijing strain. MIRU analysis demonstrated a high homogeneity of the studied collection (HGI=0.66) while 28 of 56 strains had a profile 223325153533 corresponding to Beijing/M2 subtype highly prevalent in different Russian settings. Three hypervariable loci, QUB-3232, VNTR-3820 and VNTR-4120, permitted to further subdivide 28 Beijing/M2 strains into 11 subtypes shared by 1 to 9 strains. To conclude, all markers taken together, the penitentiary population of M. tuberculosis in Kyrgyzstan exhibited a strong genetic affinity to Russia and a weak relatedness to East Asia.

Domaines: Bactériologie

Igor Mokrousov, Violeta Valcheva, Nurmira Sovhozova, Almaz Aldashev, Nalin Rastogi, et al.. Penitentiary population of Mycobacterium tuberculosis in Kyrgyzstan: exceptionally high prevalence of the Beijing genotype and its Russia-specific subtype.. Infection, Genetics and Evolution, 2009, 9 (6), pp.1400-5. ⟨10.1016/j.meegid.2009.07.007⟩. ⟨pasteur-00694590⟩