Shedding of the Pandemic Swine-Origin Influenza A Virus (H1N1) after Oseltamivir Administration

Leiyun Weng (1) , Qiang Wang (1) , Wei Wang (1) , Peijun Ren (1) , Vincent Deubel (1, 2) , Tetsuya Toyoda (1, 3, 4)

1 IPS-CAS - Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences
2 Institut Pasteur du Cambodge
3 Choju Medical Institute
4 Infectious Disease Regulation Project

We analyzed the virus shedding of an oseltamivir-treated patient who had been infected with the pandemic swine-origin influenza A (H1N1) virus which had an oseltamivir-sensitive neuraminidase. The virus was isolated from the pharyngeal swabs of the patient using MDCK cells, and the virus genome RNA was detected in the same samples both by real-time RT-PCR and RTPCR. The virus was isolated until 44 h after oseltamivir administration although the virus genome was detected until one day after oseltamivir treatment was stopped. Due to their high sensitivity, RT-PCR and real-time RT-PCR may cause misdiagnosis by detection of viral genome which does not infect, and classical virus isolation and clinical symptoms are recommended for the evaluation of oseltamivir treatment.

Domaines: Virologie

Leiyun Weng, Qiang Wang, Wei Wang, Peijun Ren, Vincent Deubel, et al.. Shedding of the Pandemic Swine-Origin Influenza A Virus (H1N1) after Oseltamivir Administration. Influenza Research and Treatment, 2010, 2010 (ID 976084), 3 p. ⟨10.1155/2010/976084⟩. ⟨pasteur-00702553⟩