Multidrug-resistant tuberculosis in Port-au-Prince, Haiti.

Abstract : OBJECTIVE: To determine the prevalence of multidrug-resistant tuberculosis (MDR-TB) among patients with new smear-positive pulmonary TB in Port-au-Prince, Haiti. METHODS: Sputum samples were cultured from 1 006 patients newly diagnosed with TB in 2008. The core region of the rpoB gene that is associated with resistance to rifampin was sequenced. All isolates with rpoB mutations were sent to the New York State reference laboratory for conventional drug susceptibility testing (DST). All isolates were also tested with the GenoType MTBDRplus line-probe assay. RESULTS: Mycobacterium tuberculosis was isolated from 906 patients. Twenty-six (2.9%) of the isolates had missense mutations or deletions in rpoB and were resistant to rifampin by DST. All 26 were also resistant to isoniazid and classified as MDR-TB. Forty-six control isolates without rpoB mutations were found to be rifampin sensitive by DST. The GenoType MTBDRplus line-probe assay correctly identified 26 MDR-TB strains. It misclassified one pansusceptible isolate as rifampin resistant. CONCLUSIONS: This study shows an MDR-TB prevalence of 2.9% in newly diagnosed TB patients in Haiti and suggests that rpoB sequencing and hybridization assays are good screening tools for early detection of MDR-TB.
Document type :
Journal articles
Complete list of metadatas

Cited literature [12 references]  Display  Hide  Download

https://hal-riip.archives-ouvertes.fr/pasteur-00708382
Contributor : Nalin Rastogi <>
Submitted on : Thursday, June 14, 2012 - 11:35:25 PM
Last modification on : Thursday, February 7, 2019 - 3:34:12 PM
Long-term archiving on : Saturday, September 15, 2012 - 2:55:50 AM

File

Reprint.pdf
Publisher files allowed on an open archive

Identifiers

  • HAL Id : pasteur-00708382, version 1
  • PUBMED : 22569696

Collections

Citation

Oksana Ocheretina, Willy Morose, Marie Gauthier, Patrice Joseph, Richard d'Meza, et al.. Multidrug-resistant tuberculosis in Port-au-Prince, Haiti.. Pan American Journal of Public Health / Revista Panamericana de Salud Pública, World Health Organization, 2012, 31 (3), pp.221-4. ⟨pasteur-00708382⟩

Share

Metrics

Record views

169

Files downloads

336