Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Autotransporter-encoding sequences are phylogenetically distributed among Escherichia coli clinical isolates and reference strains.

Abstract : Autotransporters are secreted bacterial proteins exhibiting diverse virulence functions. Various autotransporters have been identified among Escherichia coli associated with intestinal or extraintestinal infections; however, the specific distribution of autotransporter sequences among a diversity of E. coli strains has not been investigated. We have validated the use of a multiplex PCR assay to screen for the presence of autotransporter sequences. Herein, we determined the presence of 13 autotransporter sequences and five allelic variants of antigen 43 (Ag43) among 491 E. coli isolates from human urinary tract infections, diarrheagenic E. coli, and avian pathogenic E. coli (APEC) and E. coli reference strains belonging to the ECOR collection. Clinical isolates were also classified into established phylogenetic groups. The results indicated that Ag43 alleles were significantly associated with clinical isolates (93%) compared to commensal isolates (56%) and that agn43K12 was the most common and widely distributed allele. agn43 allelic variants were also phylogenetically distributed. Sequences encoding espC, espP, and sepA and agn43 alleles EDL933 and RS218 were significantly associated with diarrheagenic E. coli strains compared to other groups. tsh was highly associated with APEC strains, whereas sat was absent from APEC. vat, sat, and pic were associated with urinary tract isolates and were identified predominantly in isolates belonging to either group B2 or D of the phylogenetic groups based on the ECOR strain collection. Overall, the results indicate that specific autotransporter sequences are associated with the source and/or phylogenetic background of strains and suggest that, in some cases, autotransporter gene profiles may be useful for comparative analysis of E. coli strains from clinical, food, and environmental sources.
Document type :
Journal articles
Complete list of metadata
Contributor : Charles M. Dozois Connect in order to contact the contributor
Submitted on : Wednesday, July 25, 2012 - 5:28:05 PM
Last modification on : Monday, October 8, 2018 - 5:44:04 PM

Links full text




Concetta Restieri, Geneviève Garriss, Marie-Claude Locas, Charles M Dozois. Autotransporter-encoding sequences are phylogenetically distributed among Escherichia coli clinical isolates and reference strains.. Applied and Environmental Microbiology, American Society for Microbiology, 2007, 73 (5), pp.1553-62. ⟨10.1128/AEM.01542-06⟩. ⟨pasteur-00720799⟩



Record views