Quantitative analysis of the relative transcript levels of four chlorophenol reductive dehalogenase genes in Desulfitobacterium hafniense PCP-1 exposed to chlorophenols.

Ariane Bisaillon (1) , Réjean Beaudet (1) , François Lépine (1) , Richard Villemur (1)

1 INRS-IAF - Institut Armand Frappier

Relative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes (cprA2, cprA3, cprA4, and cprA5) in Desulfitobacterium hafniense PCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures, cprA2 and cprA3 were upregulated, as was cprA5, but concomitantly with the appearance of 2,4-dichlorophenol (DCP). In 3,5-DCP-amended cultures, only cprA5 was upregulated. In pentachlorophenol-amended cultures grown for 12 h, cprA2 and cprA3 were upregulated but not cprA5. cprA4 was not upregulated significantly in cultures containing any tested chlorophenols.

Domaines: Biotechnologies; Biotechnologie; Microbiologie et Parasitologie

Ariane Bisaillon, Réjean Beaudet, François Lépine, Richard Villemur. Quantitative analysis of the relative transcript levels of four chlorophenol reductive dehalogenase genes in Desulfitobacterium hafniense PCP-1 exposed to chlorophenols.. Applied and Environmental Microbiology, 2011, 77 (17), pp.6261-4. ⟨10.1128/AEM.00390-11⟩. ⟨pasteur-00722648⟩