Generic microtiter plate assay for triaging clinical specimens prior to genotyping of human papillomavirus DNA via consensus PCR.

Véronique Legault (1) , Ann Burchell (2, 3) , Patricia Goggin (4) , Belinda Nicolau (5) , Paul Brassard (6) , Julie Guenoun (1) , Pierre Forest (1) , Marie-Hélène Mayrand (1, 7) , Eduardo Luis Franco (3) , Francois Coutlée (1, 3, 7)

1 Laboratoire de Virologie Moléculaire
2 University of Toronto
3 Division of Cancer Epidemiology
4 Institut national de santé publique du Québec
5 INRS-IAF - Institut Armand Frappier
6 Department of Epidemiology, Biostatistics and Occupational Health [Montréal]
7 Départements de Microbiologie-Immunologie et de Gynécologie-Obstétrique

A generic human papillomavirus (HPV) probe assay was compared to the Linear Array to detect HPV DNA in 1,013 clinical specimens. The sensitivity, specificity, and negative predictive value of the assay were 99.5% (95% confidence interval [CI], 98.4% to 99.9%), 58.6% (95% CI, 53.9% to 63.1%), and 98.9% (95% CI, 96.5% to 99.8%), respectively. This assay conveniently identifies HPV-positive specimens.

Domaines: Biotechnologies; Biotechnologie

Véronique Legault, Ann Burchell, Patricia Goggin, Belinda Nicolau, Paul Brassard, et al.. Generic microtiter plate assay for triaging clinical specimens prior to genotyping of human papillomavirus DNA via consensus PCR.. Journal of Clinical Microbiology, 2011, 49 (11), pp.3977-9. ⟨10.1128/JCM.05646-11⟩. ⟨pasteur-00722655⟩