Genetic elements in the VP region of porcine parvovirus are critical to replication efficiency in cell culture. - Archive ouverte HAL Access content directly
Journal Articles Journal of Virology Year : 2011

Genetic elements in the VP region of porcine parvovirus are critical to replication efficiency in cell culture.

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Abstract

Factors controlling porcine parvovirus (PPV) replication efficiency are poorly characterized. Two prototype strains of PPV, NADL-2 and Kresse, differ greatly in pathogenic capacity both in vivo and in vitro, yet their genomic sequence is nearly identical (13 single-nucleotide substitutions and a 127-nucleotide noncoding repeated sequence). We have created a series of chimeras of these strains to identify the genetic elements involved in replication efficiency in the host porcine cell line. While the capsid proteins ultimately determine viral replication fitness, interaction between the NS1 protein and the VP gene occurs and involves interaction with the noncoding repeated sequence.

Dates and versions

pasteur-00723282 , version 1 (08-08-2012)

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Sandra Fernandes, Maude Boisvert, Peter Tijssen. Genetic elements in the VP region of porcine parvovirus are critical to replication efficiency in cell culture.. Journal of Virology, 2011, 85 (6), pp.3025-9. ⟨10.1128/JVI.02215-10⟩. ⟨pasteur-00723282⟩

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