Toll-like receptor-mediated anti-inflammatory action of glaucine and oxoglaucine.

Mimi Remichkova (1) , Petya Dimitrova (1) , Stefan Philipov (2) , Nina Ivanovska (1)

1 Institut Stephan Angeloff
2 Department of chemistry of Natural Products

Two isochinoline alkaloids, glaucine and oxoglaucine were investigated for their suggested anti-inflammatory influence concerning nitric oxide and cytokine production. Mouse peritoneal macrophages were stimulated with different Toll-like receptor (TLR) ligands such as LPS for TLR4, zymosan for TLR2 and CpG for TLR9. The alkaloids inhibited TNF-alpha and IL-6 production induced by these ligands. In regard to IL-12 suppressive effect was registered in the case of CpG stimulation. Glaucine succeeded to enhance LPS and zymosan-induced IL-10 production. The reduction of pro-inflammatory cytokines and increase of anti-inflammatory IL-10 are indicative for their use in different acute and chronic inflammatory diseases.

Keywords: Glaucine; Oxoglaucine; Toll-like receptors; Macrophages; Cytokines; Nitric oxide

Domains: Life Sciences [q-bio]; Immunology

Mimi Remichkova, Petya Dimitrova, Stefan Philipov, Nina Ivanovska. Toll-like receptor-mediated anti-inflammatory action of glaucine and oxoglaucine.. Fitoterapia, 2009, 80 (7), pp.411-4. ⟨10.1016/j.fitote.2009.05.016⟩. ⟨pasteur-00736334⟩