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Effect of vitamin E and vitamin C combination on experimental influenza virus infection.

Abstract : Successful antioxidant treatment of the so-called "free radical diseases" has been reported in the literature. In this study we examined the preventive effect of vitamin E and vitamin C, alone and in combination, on the damage caused by influenza virus infection (IVI). Male mice (ICR), infected with influenza virus A/2/68/(H3N2) (1.5 of LD(50)), were administered single once-daily doses of vitamin E (60 mg/kg b.w.) and vitamin C (80 mg/kg b.w.) intraperitoneally (3 days before virus inoculation). On the 5th and 7th day, respectively, after virus inoculation, animals were decapitated. Monooxygenase enzyme activity (ethylmorphine N-demethylase, amidopyrin N-demethylase, analgin N-demethylase, aniline hydroxylase, cytochrome P-450 content and NADPH-cytochrome C reductase [CCR]) was determined in liver 9000 x g supernatant. Primary and secondary products of lipid peroxidation (LPO; conjugated dienes [CD] and TBA-reactive substances) were measured in blood plasma, lung and liver 9000 x g supernatant. Vitamin E effectively restored LPO-levels increased by IVI. The effect of vitamin C was similar, but slighter. The combination (vitamin E + C) had greater effect on LPO levels than their separate administration. P-450-dependent monooxygenase activity was significantly restored and more pronounced cytochrome P-450 content and NADPH-CCR activity was noted. The preventive effect of vitamin E was stronger than the effect of vitamin C, but the combination (vitamin E + C) had the strongest effect. The superior protective effect of the combination is probably due to vitamin C's repairing effect on vitamin E's tocopheroxyl radical.
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Submitted on : Friday, September 28, 2012 - 11:38:45 AM
Last modification on : Tuesday, October 22, 2019 - 11:03:53 AM
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L. P. Tantcheva, E. S. Stoeva, A. S. Galabov, A. A. Braykova, V. M. Savov, et al.. Effect of vitamin E and vitamin C combination on experimental influenza virus infection.. Methods and findings in experimental and clinical pharmacology, Prous Science, 2003, 25 (4), pp.259-64. ⟨10.1358/mf.2003.25.4.769673⟩. ⟨pasteur-00736450⟩

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