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Cardiotoxin-I: an unexpectedly potent insulinotropic agent.

Abstract : Insulin secretion from pancreatic β-cells is a complex process, involving the integration and interaction of multiple external and internal stimuli, in which glucose plays a major role. Understanding the physiology leading to insulin release is a crucial step toward the identification of new targets. In this study, we evaluated the presence of insulinotropic metabolites in Naja kaouthia snake venom. Only one fraction, identified as cardiotoxin-I (CTX-I) was able to induce insulin secretion from INS-1E cells without affecting cell viability and integrity, as assessed by MTT and LDH assays. Interestingly, CTX-I was also able to stimulate insulin secretion from INS-1E cells even in the absence of glucose. Although cardiotoxins have been characterized as potent hemolytic agents and vasoconstrictors, CTX-I was unable to induce direct hemolysis of human erythrocytes or to induce potent vasoconstriction. As such, this newly identified insulin-releasing toxin will surely enrich the pool of existing tools to study β-cell physiology or even open a new therapeutic avenue.
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https://hal-riip.archives-ouvertes.fr/pasteur-00736967
Contributor : Nicolas Doucet <>
Submitted on : Monday, October 1, 2012 - 5:12:38 AM
Last modification on : Wednesday, January 29, 2020 - 1:50:20 PM

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Thi Tuyet Nhung Nguyen, Benjamin Folch, Myriam Létourneau, David Vaudry, Nam Hai Truong, et al.. Cardiotoxin-I: an unexpectedly potent insulinotropic agent.. ChemBioChem, Wiley-VCH Verlag, 2012, 13 (12), pp.1805-12. ⟨10.1002/cbic.201200081⟩. ⟨pasteur-00736967⟩

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