Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Evaluation of an enzyme-linked immunosorbent assay based on crude leishmania histone proteins for serodiagnosis of human infantile visceral leishmaniasis.

Abstract : Human visceral leishmaniasis (VL) is routinely diagnosed by detecting IgG that specifically binds to Leishmania antigens. The enzyme-linked immunosorbent assay (ELISA) remains a widely used method. However, the biggest challenge remains the choice of antigen with the highest specificity and sensitivity. This study is aimed at assessing the diagnostic performances of crude Leishmania histone (CLH) protein-based ELISAs in Mediterranean VL patients. The CLH proteins were biochemically purified from promastigote nuclear extracts. Their reactivities were analyzed by Western blotting (WB) using rabbit polyclonal antibodies against Leishmania recombinant histones and sera from VL patients, respectively. Then, the diagnostic potential of CLH proteins was validated by the CLH-based ELISA using 42 infantile VL patients' sera and 70 control subjects. The CLH-based ELISA performance was compared to that of the soluble Leishmania antigen (SLA)- and the recombinant K39 (rK39)-based ELISAs. Analysis of the WB profile with the use of polyclonal antibodies confirmed the histone origin of low molecular mass proteins (12 to 16 kDa). All VL samples tested presented antibodies reacting against different antigen fractions; however, recognition patterns were different depending on the reactivity of each serum. CLH-based ELISA showed an excellent ability to discriminate between VL cases and healthy controls (97.6% sensitivity and 100% specificity). It had a diagnostic performance similar to that of rK39-based ELISA (97.6% sensitivity and 97.1% specificity, P = 0.5) and a better serodiagnosis accuracy than the SLA-based ELISA (85.7% sensitivity and 90% specificity, P < 0.05). Therefore, crude Leishmania histone extract could be a valuable antigen for clinical use.
Document type :
Journal articles
Complete list of metadata

Cited literature [41 references]  Display  Hide  Download
Contributor : Institut Pasteur Tunis Connect in order to contact the contributor
Submitted on : Thursday, October 18, 2012 - 6:19:01 PM
Last modification on : Wednesday, October 17, 2018 - 12:44:06 PM
Long-term archiving on: : Saturday, December 17, 2016 - 2:54:56 AM


 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document




Sami Lakhal, Salima Mekki, Imène Ben-Abda, Mohamed Mousli, Fethi Amri, et al.. Evaluation of an enzyme-linked immunosorbent assay based on crude leishmania histone proteins for serodiagnosis of human infantile visceral leishmaniasis.. Clinical and Vaccine Immunology, American Society for Microbiology, 2012, 19 (9), pp.1487-91. ⟨10.1128/CVI.00257-12⟩. ⟨pasteur-00743373⟩



Record views