Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, Epiciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Fusion of HBsAg and prime/boosting augment Th1 and CTL responses to HCV polytope DNA vaccine.

Abstract : Correlation of hepatitis C virus (HCV) spontaneous resolution with Th1 and CD8(+)CTL responses during natural infection implies the potentiality of poly-CTL-epitopic HCV vaccines. We recently reported in silico design and construction of DNA vaccines (pcPOL-plasmids) harboring HCV CTL epitopes. Herein, we provide data of mice immunization by pcPOL, (encoding; core(132-142) [C], E2(405-414) [E(4)], E2(614-622) [E(6)] and NS3(1406-1415) [N] CD8(+)CTL epitopes as CE(4)E(6)N polytope) and its HBsAg-fused counterpart (pcHPOL), compared to the adjuvant-formulated (Montanide+CpG) CE(4)E(6)N synthetic-peptide immunization. All vaccinated groups developed different levels of cellular responses, however, only the pcHPOL-immunized mice elicited strong CTLs and IFN-gamma-secreting cells that were further augmented towards a Th1 response and partial tumor protection by DNA-prime/peptide-boosting regimen. Priming with HBsAg alone could not afford its augmenting effect indicating the importance of priming by polytope itself. Hence, fusion of immunocarriers like HBsAg conjoined with DNA-prime/peptide-boost immunization regimen seems a strategy to enhance the epitope-specific immune responses towards poly-CTL-epitopic vaccines.
Document type :
Journal articles
Complete list of metadata

Cited literature [28 references]  Display  Hide  Download
Contributor : Arash Memarnejadian Connect in order to contact the contributor
Submitted on : Wednesday, October 24, 2012 - 10:34:15 AM
Last modification on : Wednesday, September 23, 2020 - 10:48:02 AM
Long-term archiving on: : Saturday, December 17, 2016 - 4:10:50 AM


Files produced by the author(s)




Arash Memarnejadian, Farzin Roohvand. Fusion of HBsAg and prime/boosting augment Th1 and CTL responses to HCV polytope DNA vaccine.. Cellular Immunology, Elsevier, 2010, 261 (2), pp.93-8. ⟨10.1016/j.cellimm.2009.11.005⟩. ⟨pasteur-00744908⟩



Record views


Files downloads