Characterisation of the anti-inflammatory potential of Vitex trifolia L. (Labiatae), a multipurpose plant of the Pacific traditional medicine.

Mariko Matsui; Shilpa Kumar-Roine; H Taiana Darius; Mireille Chinain; Dominique Laurent; Serge Pauillac

Characterisation of the anti-inflammatory potential of Vitex trifolia L. (Labiatae), a multipurpose plant of the Pacific traditional medicine.

Mariko Matsui ^{1, 2, 3} Shilpa Kumar-Roine ^{2, 3} H Taiana Darius ⁴ Mireille Chinain ⁴ Dominique Laurent ^{2, 3} Serge Pauillac ^{1, *}

* Corresponding author

1 Institut Pasteur de Nouvelle-Calédonie

2 Pharmacochimie des substances naturelles et pharmacophores redox

3 IRD [Nouvelle-Calédonie] - Institut de Recherche pour le Développement

4 Laboratoire des Micro-algues toxiques

Abstract : AIM OF THE STUDY: Vitex trifolia L. (Labiatae) is a plant commonly employed against Ciguatera Fish Poisoning (CFP) in the Pacific region. Here, the anti-inflammatory potential of an aqueous extract of Vitex trifolia leaves was evaluated by monitoring its effects on the modulation of cytokines, the mediators of inflammation, as well as on the expression profiles of inducible nitric oxide synthase (iNOS) which produces the free radical nitric oxide (NO). MATERIALS AND METHODS: We prepared an aqueous extract from Vitex trifolia leaves and evaluated its anti-inflammatory potency by monitoring its effect on the lipopolysaccharide (LPS)-induced cytokines and iNOS mRNA over-production in RAW 264.7 macrophages using quantitative Polymerase Chain Reaction (qPCR) and Enzyme-Linked Immunosorbent Assay (ELISA) methods. RESULTS: Aqueous extract of Vitex trifolia leaves showed significant dose- and time-dependent inhibitory activity on interleukin (IL)-1 beta, IL-6 and iNOS mRNA synthesis, but slight effect on tumor necrosis factor (TNF)-alpha, all of which are involved in the inflammatory response. Moreover, the plant extract seemed to induce the LPS-dependent IL-10 anti-inflammatory cytokine. These results were further confirmed by ELISA using specific antibodies to mouse IL-6, IL-10 and TNF-alpha. CONCLUSION: The anti-inflammatory effects of Vitex trifolia could validate its utilization as a traditional remedy against CFP and emphasises its potential therapeutic value against other inflammatory diseases. Therefore, this plant is a promising candidate for further screening of its active compounds through activity-guided fractionation.

Keywords : Vitex trifolia Labiatae Ciguatera Fish Poisoning Lipopolysaccharide Anti-inflammatory activity Cytokines

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Human health and pathology / Infectious diseases

Complete list of metadatas

https://hal-riip.archives-ouvertes.fr/pasteur-00746410
Contributor : Mariko Matsui <>
Submitted on : Sunday, October 28, 2012 - 11:43:18 PM
Last modification on : Friday, October 11, 2019 - 8:22:44 PM

Characterisation of the anti-inflammatory potential of Vitex trifolia L. (Labiatae), a multipurpose plant of the Pacific traditional medicine.

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Characterisation of the anti-inflammatory potential of Vitex trifolia L. (Labiatae), a multipurpose plant of the Pacific traditional medicine.

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