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Transient expression assay of Agamma-588 (A/G) mutations in the K562 cell line.

Abstract : BACKGROUND: In the previous study, we have shown that the presence of A allele at position -588 in Agamma-globin gene was highly frequent and closely associated with fetal hemoglobin elevation among beta-thalassemia intermedia patients. Therefore, we decided to investigate whether this allele (A allele at -588) could result in an increase in Agamma-globin gene expression to ameliorate the severity of the disease in thalassemia patients. METHODS: Three constructs containing mu locus control region, Agamma-globin and beta-globin genes were designed and employed in the transient expression assay. The difference among constructs was in the promoter region of Agamma-globin gene (A and G alleles at -588). A construct with T to C base substitution at -175 of Agamma-globin, created by site-directed mutagenesis, was selected as positive control. The K562 cell line was transfected with the above constructs. Subsequently, the expression of Agamma-globin gene was determined by quantitative real-time reverse transcription-PCR. RESULTS: There was not a significant increase in the expression of Agamma-globin gene in the construct containing A allele comparing the one with G allele at -588. CONCLUSIONS: -588 (A>G) mutation does not play a major role in regulation of Agamma-globin gene, suggesting that other factors may be involved.
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Submitted on : Tuesday, November 20, 2012 - 8:19:16 AM
Last modification on : Wednesday, September 23, 2020 - 10:48:02 AM
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  • HAL Id : pasteur-00746421, version 1
  • PUBMED : 21725495

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Mohammad Hamid, Frouzandeh Mahjoubi, Mohammad Taghi Akbari, Hossein Khanahmad, Fatemeh Jamshidi, et al.. Transient expression assay of Agamma-588 (A/G) mutations in the K562 cell line.. Iranian Biomedical Journal, Pasteur Institute of Iran, 2011, 15 (1-2), pp.15-21. ⟨pasteur-00746421⟩

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